RNU6B, a frequent reference in miRNA expression studies, differentiates between deaths caused by hypothermia and chronic cardiac ischemia
|Author:||Kaija, Helena1; Pakanen, Lasse1,2; Porvari, Katja1,3|
1Faculty of Medicine, Research Unit of Internal Medicine, Department of Forensic Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, P.O. Box 5000, FI-90014 Oulu, Finland
2Forensic Medicine Unit, National Institute for Health and Welfare, P.O. Box 310, FI-90101 Oulu, Finland
3Faculty of Medicine, Research Unit of Cancer Research and Translational Medicine, Department of Pathology, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, P.O. Box 5000, FI-90014 Oulu, Finland
|Online Access:||PDF Full Text (PDF, 0.2 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019080623517
|Publish Date:|| 2019-08-06
Here, we tested the usefulness of small non-coding RNAs as references in quantitative RT-PCR expression analyses in hypothermia and chronic cardiac ischemia as the primary causes of death. Cq values of RNU6B, SCARNA17, SNORD25, and SNORA73A were determined from human cadaver samples of hypothermia and cardiac deaths. Average Cq values of RNU6B were higher in hypothermic and average SCARNA17 Cq values in chronic ischemic samples, but no difference in SNORD25 and SNORA73A Cq values could be seen between the groups. RNU6B expression levels were calculated using SNORD25, SNORA73A, and their combination as the reference in normalization. Expression of RNU6B, a widely used reference, was found to be significantly lower in hypothermia than in chronic cardiac ischemia. In these conditions, RNU6B is a useful marker differentiating hypothermia deaths from chronic ischemic heart disease deaths, but not a valid reference for normalization in expression studies.
International journal of legal medicine
|Volume:||Ahead of print|
|Issue:||Ahead of print|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
319 Forensic science and other medical sciences
318 Medical biotechnology
Open access funding provided by University of Oulu including Oulu University Hospital. This work was supported by the University of Oulu, Finland.
© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.