University of Oulu

Vyacheslav Kalchenko, Igor Meglinski, Anton Sdobnov, Yuri Kuznetsov, Alon Harmelin, "Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction," J. Biomed. Opt. 24(6) 060501 (31 May 2019),

Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction

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Author: Kalchenko, Vyacheslav1; Meglinski, Igor2,3,4; Sdobnov, Anton2;
Organizations: 1Weizmann Institute of Science (Israel)
2Univ. of Oulu (Finland)
3Institute of Engineering Physics for Biomedicine, National Research Nuclear Univ. MEPhI (Russian Federation)
4National Research Tomsk State Univ. (Russian Federation)
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3 MB)
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Language: English
Published: SPIE, 2019
Publish Date: 2019-08-16


Optical clearing agents (OCAs) and many chemicals are widely used in functional diagnosis of skin tissues. Numerous studies are associated with the transcutaneous diffusion of OCA in epidermal, dermal, and hypodermal tissues, which results in changing their optical properties. In addition, an objective approach that is suitable for screening the influence of utilized OCA, as well as various chemical agents, synthetics, and nanomaterials, on blood and lymph flows is highly desirable. In our study, a highly sensitive laser speckle imaging (LSI) system and fluorescent intravital microscopy (FIM) were used team-wise to inspect the acute skin vascular permeability reaction in mouse ear during the local application of OCA on the skin surface. Fluorescent contrast material administrated intravenously was used for quantitatively assessing the intensity of vascular permeability reaction and the strength of skin irritation. The obtained results suggest that a combined use of LSI and FIM is highly effective for monitoring the cutaneous vascular permeability reaction, with great potential for assessment of allergic reactions of skin in response to interactions with chemical substances.

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Series: Journal of biomedical optics
ISSN: 1083-3668
ISSN-E: 1560-2281
ISSN-L: 1083-3668
Volume: 24
Issue: 6
Article number: 060501
DOI: 10.1117/1.JBO.24.6.060501
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
Funding: Dr. Kalchenko acknowledges support of Krenter Institute (Weizmann–Staff Scientists Internal Grant Program). Professor Meglinski acknowledges partial support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005) and National Research Tomsk State University Academic D.I. Mendeleev Fund Program. Anton Sdobnov was supported by a grant from the Finnish Cultural Foundation (00180998).
Copyright information: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.