University of Oulu

Stelmashchuk, O., Zherebtsov, E., Zherebtsova, A., Kuznetsova, E., Vinokurov, A., Dunaev, A., Mamoshin, A., Snimshchikova, I., Borsukov, A., Bykov, A., Meglinski, I. (2017) Noninvasive control of the transport function of fluorescent coloured liposomal nanoparticles. Laser Physics Letters, 14 (6), 065603. doi:10.1088/1612-202X/aa6ef5

Noninvasive control of the transport function of fluorescent coloured liposomal nanoparticles

Saved in:
Author: Stelmashchuk, O.1; Zherebtsov, E.1,2; Zherebtsova, A.1;
Organizations: 1Orеl State University named after I.S. Turgenev, 95 Komsomolskaya, Orel 302026, Russia
2Aston University, Aston Institute of Photonic Technologies, Birmingham, B4 7ET, UK
3Smolensk State Medical University, 40 Krupskaya, Smolensk 214019, Russia
4Opto-Electronics and Measurement Techniques, Faculty of Information Technology and Electrical Engineering, University of Oulu, Erkki Koiso-Kanttilankatu 3, Oulu 90570, Finland
5Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Prospect Lenina 36, Tomsk 634050, Russia
6ITMO University, Kronverkskiy Prospect 49, St. Petersburg 197101, Russia
7Institute of Biology, Irkutsk State University, 3 Lenin, Irkutsk 664003, Russia
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.2 MB)
Persistent link:
Language: English
Published: IOP Publishing, 2017
Publish Date: 2019-08-22


The use of liposomal nanoparticles with an incorporated active substance is an innovative and promising approach to diagnostics and therapy. The application of liposomal nanoparticle-based drugs allows for targeted localized delivery, overcomes the natural barriers within the body effectively, and minimizes possible side effects. Liposomes are able to contain a variety of ingredients with practically no limitations to their chemical composition, chemical properties, or size of constituent molecules. This study evaluated the ability to control the passage of fluorescent dye-filled liposomes through the intestinal mucosal barrier after oral administration. For this purpose, the increase in transcutaneous registered fluorescence from tetrabromofluorescein dye was recorded and analysed. Fluorescence intensity was measured at the proximal end of the tail of an animal model after oral administration of the liposomes. Measurements were taken at the excitation wavelengths of 365 and 450 nm. The fluorescence intensity in the group treated with the fluorescent contrast agent encapsulated in liposomal particles increased 140% of the initial level, but in the group treated with pure contrast agent, the increase in detected fluorescence intensity did not exceed 110%. Mice that received empty liposomes as well as the control group did not demonstrate statistically significant changes in fluorescence intensity. A potential application of our results is an express laser optical method of monitoring the transport of orally administered liposomal particles. The results can be used to help create new optical tools for use in the development of new drugs and in high-throughput screening used during their testing.

see all

Series: Laser physics letters
ISSN: 1612-2011
ISSN-E: 1612-202X
ISSN-L: 1612-2011
Volume: 14
Issue: 6
Article number: 065603
DOI: 10.1088/1612-202X/aa6ef5
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
Funding: This work was supported by grant No. MK-7168.2016.8 from the President of the Russian Federation for state support of young Russian scientists and by RFBR grant No. 16-32-00662. I Meglinski aknowledges partial support of the Russian Science Foundation (Grant No.15-14-10008). E. Zherebtsov acknowledges for personal support H2020 MSCA-funded project No. 703145.
Copyright information: © 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at