Natural monoclonal antibody to oxidized low-density lipoprotein and Aggregatibacter actinomycetemcomitans |
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Author: | Wang, Chunguang1,2; Hörkkö, Sohvi1,2 |
Organizations: |
1Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu 90014, Finland 2Medical Research Center and Nordlab Oulu, University Hospital and University of Oulu, Oulu 90029, Finland |
Format: | article |
Version: | accepted version |
Access: | open |
Online Access: | PDF Full Text (PDF, 0.5 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2019082926063 |
Language: | English |
Published: |
Humana Press,
2017
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Publish Date: | 2019-08-29 |
Description: |
AbstractNatural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis. Surprisingly, the binding molecule of Aa bacteria to the Aa_Mab was Aa chaperonin 60 or HSP60, a protein that is not only responsible for maintaining cellular proteins conformation, but also functions as a potent virulence factor prompting bone resorption in periodontitis and as a putative pathogenic factor in atherosclerosis. see all
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Series: |
Methods in molecular biology |
ISSN: | 1064-3745 |
ISSN-E: | 1940-6029 |
ISSN-L: | 1064-3745 |
ISBN: | 978-1-4939-7180-0 |
ISBN Print: | 978-1-4939-7179-4 |
Issue: | 1643 |
Pages: | 155 - 167 |
DOI: | 10.1007/978-1-4939-7180-0_12 |
OADOI: | https://oadoi.org/10.1007/978-1-4939-7180-0_12 |
Host publication: |
Natural Antibodies : Methods and Protocols |
Host publication editor: |
Kaveri, Srinivas V. Bayry, Jagadeesh |
Type of Publication: |
A3 Book chapter |
Field of Science: |
3111 Biomedicine |
Subjects: | |
Funding: |
This study was funded by Sigrid Juselius Foundation and Finnish Foundation for Cardiovascular Research. |
Copyright information: |
© Springer Science+Business Media LLC 2017. This is a post-peer-review, pre-copyedit version of an article published in Natural Antibodies. Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-7180-0_12. |