University of Oulu

Wang C., Hörkkö S. (2017) Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans. In: Kaveri S., Bayry J. (eds) Natural Antibodies. Methods in Molecular Biology, vol 1643. Humana Press, New York, NY

Natural monoclonal antibody to oxidized low-density lipoprotein and Aggregatibacter actinomycetemcomitans

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Author: Wang, Chunguang1,2; Hörkkö, Sohvi1,2
Organizations: 1Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu 90014, Finland
2Medical Research Center and Nordlab Oulu, University Hospital and University of Oulu, Oulu 90029, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019082926063
Language: English
Published: Humana Press, 2017
Publish Date: 2019-08-29
Description:

Abstract

Natural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis. Surprisingly, the binding molecule of Aa bacteria to the Aa_Mab was Aa chaperonin 60 or HSP60, a protein that is not only responsible for maintaining cellular proteins conformation, but also functions as a potent virulence factor prompting bone resorption in periodontitis and as a putative pathogenic factor in atherosclerosis.

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Series: Methods in molecular biology
ISSN: 10643745
ISSN-E: 1940-6029
ISSN-L: 10643745
ISBN: 978-1-4939-7180-0
ISBN Print: 978-1-4939-7179-4
Issue: 1643
Pages: 155 - 167
DOI: 10.1007/978-1-4939-7180-0_12
OADOI: https://oadoi.org/10.1007/978-1-4939-7180-0_12
Host publication: Natural Antibodies : Methods and Protocols
Host publication editor: Kaveri, Srinivas V.
Bayry, Jagadeesh
Type of Publication: A3 Book chapter
Field of Science: 3111 Biomedicine
Subjects:
Funding: This study was funded by Sigrid Juselius Foundation and Finnish Foundation for Cardiovascular Research.
Copyright information: © Springer Science+Business Media LLC 2017. This is a post-peer-review, pre-copyedit version of an article published in Natural Antibodies. Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-7180-0_12.