University of Oulu

Avsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2019). Mutual interaction of red blood cells influenced by nanoparticles. Scientific Reports, 9(1).

Mutual interaction of red blood cells influenced by nanoparticles

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Author: Avsievich, Tatiana1; Popov, Alexey1; Bykov, Alexander1;
Organizations: 1Opto-Electronics and Measurement Techniques, University of Oulu, P.O. Box 4500, Oulu, 90014, Finland
2Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, 634050, Russia
3National Research Nuclear University – MEPhI, Institute of Engineering Physics for Biomedicine (PhysBio), Moscow, 115409, Russia
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.6 MB)
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Language: English
Published: Springer Nature, 2019
Publish Date: 2019-09-05


Despite extensive studies on different types of nanoparticles as potential drug carriers, the application of red blood cells (RBCs) as natural transport agents for systemic drug delivery is considered a new paradigm in modern medicine and possesses great potential. There is a lack of studies on the influence of drug carriers of different compositions on RBCs, especially regarding their potential impact on human health. Here, we apply conventional microscopy to observe the formation of RBC aggregates and optical tweezers to quantitatively assess the mutual interaction of RBCs incubated with inorganic and polymeric nanoparticles. Scanning electron microscopy is utilized for direct observation of nanoparticle localization on RBC membranes. The experiments are performed in a platelet-free blood plasma mimicking the RBC natural environment. We show that nanodiamonds influence mutual RBC interactions more antagonistically than other nanoparticles, resulting in higher aggregation forces and the formation of larger cell aggregates. In contrast, polymeric particles do not cause anomalous RBC aggregation. The results emphasize the application of optical tweezers for the direct quantitative assessment of the mutual interaction of RBCs influenced by nanomaterials.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 9
Article number: 5147
DOI: 10.1038/s41598-019-41643-x
Type of Publication: A1 Journal article – refereed
Field of Science: 221 Nanotechnology
217 Medical engineering
Funding: The research was partially supported by EDUFI Fellowships (TM-17-10370, TM-18-10820) and the Academy of Finland (projects 290596, 314369 and 311698). IM also acknowledges partial support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005) and National Research Tomsk State University Academic D.I. Mendeleev Fund Program.
Academy of Finland Grant Number: 290596
Detailed Information: 290596 (Academy of Finland Funding decision)
314369 (Academy of Finland Funding decision)
311698 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit