Mutual interaction of red blood cells influenced by nanoparticles
|Author:||Avsievich, Tatiana1; Popov, Alexey1; Bykov, Alexander1;|
1Opto-Electronics and Measurement Techniques, University of Oulu, P.O. Box 4500, Oulu, 90014, Finland
2Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, 634050, Russia
3National Research Nuclear University – MEPhI, Institute of Engineering Physics for Biomedicine (PhysBio), Moscow, 115409, Russia
|Online Access:||PDF Full Text (PDF, 1.6 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019090526952
|Publish Date:|| 2019-09-05
Despite extensive studies on different types of nanoparticles as potential drug carriers, the application of red blood cells (RBCs) as natural transport agents for systemic drug delivery is considered a new paradigm in modern medicine and possesses great potential. There is a lack of studies on the influence of drug carriers of different compositions on RBCs, especially regarding their potential impact on human health. Here, we apply conventional microscopy to observe the formation of RBC aggregates and optical tweezers to quantitatively assess the mutual interaction of RBCs incubated with inorganic and polymeric nanoparticles. Scanning electron microscopy is utilized for direct observation of nanoparticle localization on RBC membranes. The experiments are performed in a platelet-free blood plasma mimicking the RBC natural environment. We show that nanodiamonds influence mutual RBC interactions more antagonistically than other nanoparticles, resulting in higher aggregation forces and the formation of larger cell aggregates. In contrast, polymeric particles do not cause anomalous RBC aggregation. The results emphasize the application of optical tweezers for the direct quantitative assessment of the mutual interaction of RBCs influenced by nanomaterials.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
217 Medical engineering
The research was partially supported by EDUFI Fellowships (TM-17-10370, TM-18-10820) and the Academy of Finland (projects 290596, 314369 and 311698). IM also acknowledges partial support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005) and National Research Tomsk State University Academic D.I. Mendeleev Fund Program.
|Academy of Finland Grant Number:||
290596 (Academy of Finland Funding decision)
314369 (Academy of Finland Funding decision)
311698 (Academy of Finland Funding decision)
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