University of Oulu

Han, S.-K., Ronkainen, A. P., Saarakkala, S., Rieppo, L., Herzog, W., & Korhonen, R. K. (2017). Alterations in structural macromolecules and chondrocyte deformations in lapine retropatellar cartilage 9 weeks after anterior cruciate ligament transection. Journal of Orthopaedic Research. https://doi.org/10.1002/jor.23650

Alterations in structural macromolecules and chondrocyte deformations in lapine retropatellar cartilage 9 weeks after anterior cruciate ligament transection

Saved in:
Author: Han, Sang-Kuy1,2; Ronkainen, Ari P.3; Saarakkala, Simo4;
Organizations: 1Human Performance Laboratory, University of Calgary, Calgary, Canada
2Advanced Biomedical and Welfare Technology R&BD Group, Korea Institute of Industrial Technology, Cheonan-si, Korea
3Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
4Faculty of Medicine, Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019092329407
Language: English
Published: John Wiley & Sons, 2018
Publish Date: 2019-09-23
Description:

Abstract

The structural integrity and mechanical environment of the articular cartilage matrix directly affect chondrocytedeformations. Rabbit models of early osteoarthritis at 9 weeks following anterior cruciate ligament transection (ACLT) have beenshown to alter the deformation behavior of superficial zone chondrocytes in mechanically loaded articular cartilage. However, it is notfully understood whether these changes in cell mechanics are caused by changes in structural macromolecules in the extracellularmatrix. Therefore, the purpose of this study was to characterize the proteoglycan content, collagen content, and collagen orientation at9 weeks post ACLT using microscopic techniques, and relate these changes to the altered cell mechanics observed upon mechanicalloading of cartilage. At 9 weeks following ACLT, collagen orientation was significantly (p<0.05) altered and proteoglycan content wassignificantly (p<0.05) reduced in the superficial zone cartilage matrix. These structural changes either in the extracellular orpericellular matrix (ECM and PCM) were also correlated significantly (p<0.05) with chondrocyte width and height changes, therebysuggesting that chondrocyte deformation response to mechanical compression in early OA changes primarily because of alterations inmatrix structure. However, compared to the normal group, proteoglycan content in the PCM from the ACLT group decreased less thanthat in the surrounding ECM. Therefore, PCM could play a key role to protect excessive chondrocyte deformations in the ACLT group.

see all

Series: Journal of orthopaedic research
ISSN: 0736-0266
ISSN-E: 1554-527X
ISSN-L: 0736-0266
Volume: 36
Issue: 1
Pages: 342 - 350
DOI: 10.1002/jor.23650
OADOI: https://oadoi.org/10.1002/jor.23650
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
3126 Surgery, anesthesiology, intensive care, radiology
Subjects:
Funding: This study was supported by a grant from the Korea Institute of Industrial Technology (to Dr. Han), the Academy of Finland (project 286526 to Dr. Korhonen), European Research Council (project 281180 to Dr. Korhonen), Sigrid Juselius Foundation, AI-HS Team Grant in OA (to Dr. Herzog), the Canada Research Chair Programme for Molecular and Cellular Biomechanics (to Dr. Herzog) and the Killam Memorial Chair (Dr. Herzog) at the University of Calgary, and a Foundation scheme grant from the Canadian Institutes of Health Research (Dr. Herzog).
Copyright information: © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Han, S.-K., Ronkainen, A. P., Saarakkala, S., Rieppo, L., Herzog, W., & Korhonen, R. K. (2017). Alterations in structural macromolecules and chondrocyte deformations in lapine retropatellar cartilage 9 weeks after anterior cruciate ligament transection. Journal of Orthopaedic Research. https://doi.org/10.1002/jor.23650, which has been published in final form at https://doi.org/10.1002/jor.23650. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.