University of Oulu

Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. https://doi.org/10.1002/bit.26831

A fully online sensor-equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications

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Author: Maldonado, Susanna Lladó1,2; Panjan, Peter3; Sun, Shiwen4;
Organizations: 1Institute of Biochemical Engineering, Technische Universität Braunschweig, Braunschweig, Germany
2Center of Pharmaceutical Engineering (PVZ), Technische Universität Braunschweig, Braunschweig, Germany
3Unit of Measurement Technologies, University of Oulu, Kajaani, Finland
4Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019093030475
Language: English
Published: John Wiley & Sons, 2019
Publish Date: 2019-09-10
Description:

Abstract

A new disposable, multiphase, microbioreactor (MBR; with a working volume of 550 μl) equipped with online sensors is presented for biotechnological screening research purposes owing to its high‐throughput potential. Its design and fabrication, online sensor integration, and operation are described. During aerobic cultivation, sufficient oxygen supply is the most important factor that influences growth and product formation. The MBR is a microbubble column bioreactor (μBC), and the oxygen supply was realized by active pneumatic bubble aeration, ensuring sufficient volumetric liquid‐phase mass transfer (kLa) and proper homogenization of the cultivation broth. The μBC was equipped with miniaturized sensors for the pH, dissolved oxygen, optical density and glucose concentration that allowed real‐time online monitoring of these process variables during cultivation. The challenge addressed here was the integration of sensors in the limited available space. The MBR was shown to be a suitable screening platform for the cultivation of biological systems. Batch cultivations of Saccharomyces cerevisiae were performed to observe the variation in the process variables over time and to show the robustness and operability of all the online sensors in the MBR.

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Series: Biotechnology and bioengineering
ISSN: 0006-3592
ISSN-E: 1097-0290
ISSN-L: 0006-3592
Volume: 116
Issue: 1
Pages: 65 - 75
DOI: 10.1002/bit.26831
OADOI: https://oadoi.org/10.1002/bit.26831
Type of Publication: A1 Journal article – refereed
Field of Science: 220 Industrial biotechnology
Subjects:
Funding: The authors gratefully acknowledge the financial support provided by the People Programme (Marie Curie Actions, Multi‐ITN) of the European Union’s Seventh Framework Programme for research, technological development and demonstration within the project EUROMBR—European network for innovative microbioreactor applications in bioprocess development (Project ID 608104).
EU Grant Number: (608104) EUROMBR - European network for innovative microbioreactor applications in bioprocess development
Copyright information: © 2018 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75, which has been published in final form at Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.