University of Oulu

Paarnio, K, Tuomisto, A, Väyrynen, SA, Väyrynen, JP, Klintrup, K, Ohtonen, P, Mäkinen, MJ, Mäkelä, J, Karttunen, TJ. Serum TLR2 and TLR4 levels in colorectal cancer and their association with systemic inflammatory markers, tumor characteristics, and disease outcome. APMIS 2019; 127: 561– 569.

Serum TLR2 and TLR4 levels in colorectal cancer and their association with systemic inflammatory markers, tumor characteristics, and disease outcome

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Author: Paarnio, Karoliina1,2; Tuomisto, Anne3,4; Väyrynen, Sara A.3,4,5;
Organizations: 1Research Unit of Surgery, Anesthesia and Intensive Care, University of Oulu
2Department of Surgery, Oulu University Hospital and Medical Research Center Oulu
3Cancer and Translational Medicine Research Unit, University of Oulu
4Department of Pathology, Oulu University Hospital and Medical Research Center Oulu, Oulu, Finland
5Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA
6Department of Oncologic Pathology Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2019100130656
Language: English
Published: John Wiley & Sons, 2019
Publish Date: 2020-05-27
Description:

Abstract

Toll‐like receptors (TLRs) are involved in colorectal cancer (CRC) pathogenesis. However, the significance of serum TLR concentrations in CRC is unknown. We analyzed serum TLR2 and TLR4 concentrations with ELISA in preoperative samples from 118 patients with CRC and 88 matched controls. We also assessed tissue TLR expression with immunohistochemistry and by detecting serum determinants of systemic inflammation. Most participants (>70%) had undetectable serum TLR2. The mean serum TLR4 levels were lower in patients than in controls (1.1 vs 1.8 ng/mL; p = 0.015). Undetectable TLR4 was more common in stage I (39%) than in stages II–IV (11%, p < 0.001). TLR2 or TLR4 expression in tumor cells did not correlate with serum levels, but abundant TLR2 expression in normal colon epithelium was associated with detectable serum TLR2 (p = 0.034). Undetectable serum TLR2 was linked to high modified Glasgow prognostic scores (p = 0.010), high CRP levels (p = 0.013), blood vessel invasion (p = 0.013), and tended to be associated with worse 5‐year survival (p = 0.052). In conclusion, serum TLR2 levels were inversely associated with systemic inflammation in patients with CRC. Moreover, serum TLR2 levels might depend more on normal colorectal mucosa contributions than on tumor tissue contributions. Further studies are required to assess the prognostic value of serum TLR2.

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Series: APMIS
ISSN: 0903-4641
ISSN-E: 1600-0463
ISSN-L: 0903-4641
Volume: 127
Issue: 8
Pages: 561 - 569
DOI: 10.1111/apm.12971
OADOI: https://oadoi.org/10.1111/apm.12971
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Copyright information: © 2019 APMIS. Published by John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Paarnio, K, Tuomisto, A, Väyrynen, SA, Väyrynen, JP, Klintrup, K, Ohtonen, P, Mäkinen, MJ, Mäkelä, J, Karttunen, TJ. Serum TLR2 and TLR4 levels in colorectal cancer and their association with systemic inflammatory markers, tumor characteristics, and disease outcome. APMIS 2019; 127: 561– 569., which has been published in final form at https://doi.org/10.1111/apm.12971. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.