University of Oulu

Hanna Virpiranta, Sanna Taskila, Tiina Leiviskä, Jaakko Rämö, Juha Tanskanen, Development of a process for microbial sulfate reduction in cold mining waters – Cold acclimation of bacterial consortia from an Arctic mining district, Environmental Pollution, Volume 252, Part A, 2019, Pages 281-288, ISSN 0269-7491,

Development of a process for microbial sulfate reduction in cold mining waters : cold acclimation of bacterial consortia from an Arctic mining district

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Author: Virpiranta, Hanna1; Taskila, Sanna1; Leiviskä, Tiina1;
Organizations: 1University of Oulu, Chemical Process Engineering, PO Box 4300, 90014, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.2 MB)
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Language: English
Published: Elsevier, 2019
Publish Date: 2019-10-02


Biological sulfate removal is challenging in cold climates due to the slower metabolism of mesophilic bacteria; however, cold conditions also offer the possibility to isolate bacteria that have adapted to low temperatures. The present research focused on the cold acclimation and characterization of sulfate-reducing bacterial (SRB) consortia enriched from an Arctic sediment sample from northern Finland. Based on 16S rDNA analysis, the most common sulfate-reducing bacterium in all enriched consortia was Desulfobulbus, which belongs to the δ-Proteobacteria. The majority of the cultivated consortia were able to reduce sulfate at temperatures as low as 6 °C with succinic acid as a carbon source. The sulfate reduction rates at 6 °C varied from 13 to 42 mg/L/d. The cultivation medium used in this research was a Postgate medium supplemented with lactate, ethanol or succinic acid. The obtained consortia were able to grow with lactate and succinic acid but surprisingly not with ethanol. Enriched SRB consortia are useful for the biological treatment of sulfate-containing industrial wastewaters in cold conditions.

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Series: Environmental pollution
ISSN: 0269-7491
ISSN-E: 1873-6424
ISSN-L: 0269-7491
Volume: 252
Issue: Part A
Pages: 281 - 288
DOI: 10.1016/j.envpol.2019.05.087
Type of Publication: A1 Journal article – refereed
Field of Science: 219 Environmental biotechnology
Funding: The study was conducted as part of the Comprehensive Sulfate Management in the Cold Mining Waters (COSUMA) project (grant ID 295050), funded by the Academy of Finland. Marko Suokas, M.Sc., at the Biocenter Oulu Sequencing Center is acknowledged for the 16S rDNA sequencing.
Academy of Finland Grant Number: 295050
Detailed Information: 295050 (Academy of Finland Funding decision)
Copyright information: © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (