University of Oulu

Kashif M, Jurvansuu J, Vainio EJ and Hantula J (2019) Alphapartitiviruses of Heterobasidion Wood Decay Fungi Affect Each Other's Transmission and Host Growth. Front. Cell. Infect. Microbiol. 9:64. doi: 10.3389/fcimb.2019.00064

Alphapartitiviruses of Heterobasidion wood decay fungi affect each other’s transmission and host growth

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Author: Kashif, Muhammad1; Jurvansuu, Jaana2; Vainio, Eeva J.1;
Organizations: 1Forest Health and Biodiversity, Natural Resources Institute Finland, Helsinki, Finland
2Department of Biology, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019100731436
Language: English
Published: Frontiers Media, 2019
Publish Date: 2019-10-07
Description:

Abstract

Heterobasidion spp. root rot fungi are highly destructive forest pathogens of the northern boreal forests, and are known to host a diverse community of partitiviruses. The transmission of these mycoviruses occurs horizontally among host strains via mycelial anastomoses. We revealed using dual cultures that virus transmission rates are affected by pre-existing virus infections among two strains of H. annosum. The transmission efficacy of mycovirus HetPV15-pa1 to a pre-infected host was elevated from zero to 50% by the presence of HetPV13-an1, and a double infection of these viruses in the donor resulted in an overall transmission rate of 90% to a partitivirus-free recipient. On contrary, pre-existing virus infections of two closely related strains of HetPV11 hindered each other’s transmission, but had unexpectedly dissimilar effects on the transmission of more distantly related viruses. The co-infection of HetPV13-an1 and HetPV15-pa1 significantly reduced host growth, whereas double infections including HetPV11 strains had variable effects. Moreover, the results showed that RdRp transcripts are generally more abundant than capsid protein (CP) transcripts and the four different virus strains express unique transcripts ratios of RdRp and CP. Taken together, the results show that the interplay between co-infecting viruses and their host is extremely complex and highly unpredictable.

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Series: Frontiers in microbiology
ISSN: 1664-302X
ISSN-E: 1664-302X
ISSN-L: 1664-302X
Volume: 9
Article number: 64
DOI: 10.3389/fcimb.2019.00064
OADOI: https://oadoi.org/10.3389/fcimb.2019.00064
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Subjects:
Funding: This work was supported by the Academy of Finland (grant numbers 258520 and 309896). Institution Natural Resources Institute Finland provides funds to pay fees for this open access publication.
Copyright information: © 2019 Kashif, Jurvansuu, Vainio and Hantula. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
  https://creativecommons.org/licenses/by/4.0/