University of Oulu

Rantala, S., Kaseva, J., Karhu, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 109.

Cryopreservation of Ribes nigrum (L.) dormant buds : recovery via in vitro culture to the field

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Author: Rantala, Saija1,2; Kaseva, Janne3; Karhu, Saila4;
Organizations: 1Production Systems, Natural Resources Institute Finland (Luke), Survontie 9A, 40500 Jyväskylä, Finland
2University of Oulu, Faculty of Science, Ecology and Genetics Unit, P.O.Box 3000, 90014 Oulu, Finland
3Natural Resources, Natural Resources Institute Finland (Luke), Tietotie 4, 31600 Jokioinen, Finland
4Production Systems, Natural Resources Institute Finland (Luke), Itäinen Pitkäkatu 4 A, 20520 Turku, Finland
5Boreal Plant Breeding Ltd., Myllytie 10, 31600 Jokioinen, Finland
6MTT Agrifood Research Finland, Antinniementie 1, 41330 Vihtavuori, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1 MB)
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Language: English
Published: Springer Nature, 2019
Publish Date: 2019-10-07


Cryopreservation of dormant buds can be a feasible method for long term preservation of clonally propagated woody plants. In the present study, dormant buds of blackcurrant (Ribes nigrum L) cultivar Mortti were cryopreserved. Twig segments from greenhouse and field grown plants were cooled at their natural moisture content at the rate of 0.17 °C min⁻¹ from zero to −38 °C, immersed in liquid nitrogen and stored in liquid nitrogen vapor. The post-cryopreservation regrowth of buds was evaluated in vitro and viability of recovered plants was tested in vivo. The estimated recovery rate for buds thawed after 1–6 days in cryostorage was 86% and 66% for outdoor and greenhouse grown buds, respectively. Microplants adequate in size and quality grew successfully in vivo and plants produced berries in field. After 4 years in cryostorage, the estimated recovery rate for outdoor buds was 58%. According to results from 1 to 6 days cryostorage, the tested dormant bud protocol turned to be applicable for cryopreservation of the blackcurrant cultivar Mortti. However, concerning the long-term preservation the decline of recovery is important to consider in the future experiments.

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Series: Plant cell, tissue and organ culture
ISSN: 0167-6857
ISSN-E: 1573-5044
ISSN-L: 0167-6857
Volume: 138
Issue: 1
Pages: 109 - 119
DOI: 10.1007/s11240-019-01607-5
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
1183 Plant biology, microbiology, virology
1184 Genetics, developmental biology, physiology
Funding: Open access funding provided by Natural Resources Institute Finland (LUKE). This research was partly funded by the European Commission, Directorate-General for Agriculture and Rural Development, under Council Regulation (EC) No 870/2004 through Action 071 AGRI GEN RES 870/2004 (RIBESCO) and partly by The Ministry of Agriculture and Forestry, Maiju & Yrjö Rikalan Puutarhasäätiö and Oiva Kuusisto Säätiö.
Copyright information: © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.