Pekkanen-Mattila, M.; Häkli, M.; Pölönen, R.-P.; Mansikkala, T.; Junnila, A.; Talvitie, E.; Koivisto, J.T.; Kellomäki, M.; Aalto-Setälä, K. Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Materials 2019, 12, 1805. https://doi.org/10.3390/ma12111805
Polyethylene terephthalate textiles enhance the structural maturation of human induced pluripotent stem cell-derived cardiomyocytes
|Author:||Pekkanen-Mattila, Mari1; Häkli, Martta1; Pölönen, Risto-Pekka1;|
1BioMediTech, Faculty of Medicine and Health Technology, Tampere University, 33140 Tampere, Finland
2Microelectronics Research Unit, University of Oulu, FI-90014 Oulu, Finland
3Finland and Heart Hospital, Tampere University Hospital, 33100 Tampere, Finland
|Online Access:||PDF Full Text (PDF, 1.2 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019101032188
Multidisciplinary Digital Publishing Institute,
|Publish Date:|| 2019-10-10
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult cardiomyocytes. Putative factors enhancing maturation include in vitro culture duration, culture surface topography, and mechanical, chemical, and electrical stimulation. Stem cell-derived cardiomyocytes are traditionally cultured on glass surfaces coated with extracellular matrix derivatives such as gelatin. hiPSC-CMs are flat and round and their sarcomeres are randomly distributed and unorganized. Morphology can be enhanced by culturing cells on surfaces providing topographical cues to the cells. In this study, a textile based-culturing method used to enhance the maturation status of hiPSC-CMs is presented. Gelatin-coated polyethylene terephthalate (PET)-based textiles were used as the culturing surface for hiPSC-CMs and the effects of the textiles on the maturation status of the hiPSC-CMs were assessed. The hiPSC-CMs were characterized by analyzing their morphology, sarcomere organization, expression of cardiac specific genes, and calcium handling. We show that the topographical cues improve the structure of the hiPSC-CMs in vitro. Human iPSC-CMs grown on PET textiles demonstrated improved structural properties such as rod-shape structure and increased sarcomere orientation.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
216 Materials engineering
217 Medical engineering
This research was funded by Business Finland, Päivikki and Sakari Sohlberg foundation, Pirkanmaa Regional Fund of the Finnish Cultural Foundation, the Finnish Foundation for Cardiovascular Research, Academy of Finland and Pirkanmaa Hospital District.
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).