University of Oulu

Åström P., Heljasvaara R., Nyberg P., Al-Samadi A., Salo T. (2018) Human Tumor Tissue-Based 3D In Vitro Invasion Assays. In: Cal S., Obaya A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY.

Human tumor tissue-based 3D in vitro invasion assays

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Author: Åström, Pirjo1,2; Heljasvaara, Ritva3,4; Nyberg, Pia5,6,7;
Organizations: 1Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
2Medical Research Center, Oulu University Hospital, Oulu, Finland
3Oulu Center for Cell-Matrix Research, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
4Department of Biomedicine, Centre for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway
5Biobank Borealis of Northern Finland, Oulu University Hospital, Oulu, Finland
6Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland
7Institute of Cancer Research and Translational Medicine, University of Oulu, Oulu, Finland
8Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, Helsinki, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.6 MB)
Persistent link:
Language: English
Published: Humana Press, 2018
Publish Date: 2020-01-10


Here we describe a protocol to utilize human benign leiomyoma tissue in in vitro 3D model that enables an assessment of cell invasion. The chapter also describes detailed instructions for image analysis to quantify the results. Leiomyoma is a benign tumor of the uterus which mimics authentic components of the tumor microenvironment including fibroblasts, vessels, collagen fibers, and extracellular protein composition. The leiomyoma invasion model represents a superior 3D model for cell invasion studies compared to the other non-human organotypic models.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-4939-7595-2
ISBN Print: 978-1-4939-7594-5
Pages: 213 - 221
DOI: 10.1007/978-1-4939-7595-2_19
Host publication: Proteases and Cancer : Methods and Protocols
Host publication editor: Cal, Santiago
Obaya, Alvaro J.
Type of Publication: A3 Book chapter
Field of Science: 3122 Cancers
Funding: This work was supported by Sigrid Juselius Foundation, the Finnish Cancer Foundation, the University of Helsinki Central Hospital, and the Health Science Council of the Academy of Finland (Centre of Excellence 2012–2017 Grant 251314). We thank Dr. Sini Nurmenniemi and Dr. Tatu Pirkola for developing the myoma model and the protocol of invasion quantification, respectively. We thank Mr. Donald Smart for the editorial assistance.
Academy of Finland Grant Number: 251314
Detailed Information: 251314 (Academy of Finland Funding decision)
Copyright information: © Springer Science+Business Media, LLC 2018. This is a post-peer-review, pre-copyedit version of an book chapter published in Proteases and Cancer (ISBN: 978-1-4939-7594-5). The final authenticated version is available online at: