University of Oulu

Monteiro, C., Costa, F., Pirttilä, A., Tejesvi, M., Martins, M. (2019) Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes. Scientific reports 9(1), 10753. doi:10.1038/s41598-019-47108-5

Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes

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Author: Monteiro, Claudia1,2; Fabíola Costa, Fabíola Costa1,2; Pirttilä, Anna Maria3;
Organizations: 1i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
2INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal
3Department of Ecology and Genetics, University of Oulu, Oulu, Finland
4CHAIN ANTIMICROBIALS ltd, Teknologiantie 2, Oulu, 90590, Finland
5Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2 MB)
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Language: English
Published: Springer Nature, 2019
Publish Date: 2019-10-29


Urinary catheters are extensively used in hospitals, being responsible for about 75% of hospital-acquired infections. In this work, a de novo designed antimicrobial peptide (AMP) Chain201D was studied in the context of urinary catheter-associated infections. Chain201D showed excellent antimicrobial activity against relevant ATCC strains and clinical isolates of bacteria and yeast and demonstrated high stability in a wide range of temperatures, pH and salt concentrations. Moreover, the bactericidal activity of Chain201D immobilized on a model surface was studied against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), some of the most prevalent strains found in urinary catheter-associated infections. Chain201D was successfully tethered to ((1-mercapto-11-undecyl)-(tetra(ethylene glycol) (EG4)) terminated self-assembled monolayers (SAMs), (EG4-SAMs), activated by 1,1′-Carbonyldiimidazole (CDI) at different concentrations. Chain201D surfaces can bind and kill by contact a high percentage of adherent bacteria. These achievements are obtained without any peptide modification (for chemoselective conjugation) and without the use of a spacer. Moreover, increased amounts of immobilized AMP lead to higher numbers of adhered/dead bacteria, revealing a concentration-dependent behaviour and demonstrating that Chain201D has excellent potential for developing antimicrobial urinary catheters.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 9
Article number: 10753
DOI: 10.1038/s41598-019-47108-5
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
Funding: AntINFECT: Bioengineered Advanced Therapies for Problematic Infected Wounds POCI-01-0145-FEDER-031781) and Institute for Research and Innovation in Health Sciences (UID/BIM/04293/2019). C. Monteiro would like to acknowledge national funding through FCT – Fundação para a Ciência e a Tecnologia, I.P., provided by the contract-program and according to numbers 4, 5 and 6 of art. 23 of Law no. 57/2016 of 29th August, as amended by Law no. 57/2017 of 19th July. Professor Paula Gomes from Faculdade de Ciências da Universidade do Porto (FC-UP), Porto Peptide Synthesis Facility (POP-UP) for CEM 7 peptide. The funding received from Tekes, Finland (3331/31/2014) is thankfully acknowledged.
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