Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes |
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Author: | Hujaya, Sry D.1; Manninen, Aki2; Kling, Kirsten3; |
Organizations: |
1Fibre and Particle Engineering Research Unit, University of Oulu, P.O. Box 4300, FI-90014 Oulu, Finland 2Center for Cell-Matrix Research, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014, Finland 3National Research Centre for the Working Environment, Technical University of Denmark, Lersø Parkallé 105, Copenhagen DK-2100, Denmark
4Laboratory of Developmental Biology, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014 Oulu, Finland
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Format: | article |
Version: | accepted version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.9 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2019103035926 |
Language: | English |
Published: |
Elsevier,
2019
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Publish Date: | 2021-06-03 |
Description: |
AbstractCellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials. see all
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Series: |
Journal of colloid and interface science |
ISSN: | 0021-9797 |
ISSN-E: | 1095-7103 |
ISSN-L: | 0021-9797 |
Volume: | 553 |
Pages: | 71 - 82 |
DOI: | 10.1016/j.jcis.2019.06.001 |
OADOI: | https://oadoi.org/10.1016/j.jcis.2019.06.001 |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
1182 Biochemistry, cell and molecular biology |
Subjects: | |
Funding: |
This work was supported by the Academy of Finland (NanoBioMass project 307535). |
Academy of Finland Grant Number: |
307535 |
Detailed Information: |
307535 (Academy of Finland Funding decision) |
Copyright information: |
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |