University of Oulu

Sry D. Hujaya, Aki Manninen, Kirsten Kling, Jakob B. Wagner, Seppo J. Vainio, Henrikki Liimatainen, Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes, Journal of Colloid and Interface Science, Volume 553, 2019, Pages 71-82, ISSN 0021-9797, https://doi.org/10.1016/j.jcis.2019.06.001

Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes

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Author: Hujaya, Sry D.1; Manninen, Aki2; Kling, Kirsten3;
Organizations: 1Fibre and Particle Engineering Research Unit, University of Oulu, P.O. Box 4300, FI-90014 Oulu, Finland
2Center for Cell-Matrix Research, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014, Finland
3National Research Centre for the Working Environment, Technical University of Denmark, Lersø Parkallé 105, Copenhagen DK-2100, Denmark
4Laboratory of Developmental Biology, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014 Oulu, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2019103035926
Language: English
Published: Elsevier, 2019
Publish Date: 2021-06-03
Description:

Abstract

Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.

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Series: Journal of colloid and interface science
ISSN: 0021-9797
ISSN-E: 1095-7103
ISSN-L: 0021-9797
Volume: 553
Pages: 71 - 82
DOI: 10.1016/j.jcis.2019.06.001
OADOI: https://oadoi.org/10.1016/j.jcis.2019.06.001
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
RGD
Funding: This work was supported by the Academy of Finland (NanoBioMass project 307535).
Academy of Finland Grant Number: 307535
Detailed Information: 307535 (Academy of Finland Funding decision)
Copyright information: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/