Sry D. Hujaya, Aki Manninen, Kirsten Kling, Jakob B. Wagner, Seppo J. Vainio, Henrikki Liimatainen, Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes, Journal of Colloid and Interface Science, Volume 553, 2019, Pages 71-82, ISSN 0021-9797, https://doi.org/10.1016/j.jcis.2019.06.001
Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes
|Author:||Hujaya, Sry D.1; Manninen, Aki2; Kling, Kirsten3;|
1Fibre and Particle Engineering Research Unit, University of Oulu, P.O. Box 4300, FI-90014 Oulu, Finland
2Center for Cell-Matrix Research, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014, Finland
3National Research Centre for the Working Environment, Technical University of Denmark, Lersø Parkallé 105, Copenhagen DK-2100, Denmark
4Laboratory of Developmental Biology, Biocenter Oulu, University of Oulu, P.O. Box 5000, FI-90014 Oulu, Finland
|Online Access:||PDF Full Text (PDF, 1.9 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019103035926
|Publish Date:|| 2021-06-03
Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.
Journal of colloid and interface science
|Pages:||71 - 82|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1182 Biochemistry, cell and molecular biology
This work was supported by the Academy of Finland (NanoBioMass project 307535).
|Academy of Finland Grant Number:||
307535 (Academy of Finland Funding decision)
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.