University of Oulu

Tóth, F. , Johnson, C. P., Mills, B. , Nissi, M. J., Nykänen, O. , Ellermann, J. , Ludwig, K. D., Tompkins, M. and Carlson, C. S. (2019), Evaluation of the Suitability of Miniature Pigs as an Animal Model of Juvenile Osteochondritis Dissecans. J. Orthop. Res., 37: 2130-2137. doi:10.1002/jor.24353

Evaluation of the suitability of miniature pigs as an animal model of juvenile osteochondritis dissecans

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Author: Tóth, Ferenc1; Johnson, Casey P.2; Mills, Benigno1;
Organizations: 1Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota
2Department of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, Minnesota
3Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
4Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland
5Department of Orthopedic Surgery, University of Minnesota, Minneapolis, Minnesota
6Department of Veterinary Clinical Sciences, University of Minnesota, St. Paul, Minnesota
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019112043231
Language: English
Published: John Wiley & Sons, 2019
Publish Date: 2020-05-22
Description:

Abstract

Juvenile osteochondritis dissecans (JOCD) is a developmental disease characterized by formation of intra‐articular (osteo)chondral flaps or fragments. Evidence‐based treatment guidelines for JOCD are currently lacking. An animal model would facilitate study of JOCD and evaluation of diagnostic and treatment approaches. The purpose of this study was to assess the suitability of miniature pigs as a model of JOCD at the distal femur. First, stifle (knee) joints harvested from three juvenile miniature pigs underwent magnetic resonance imaging (MRI) to establish the vascular architecture of the distal femoral epiphyseal cartilage. Second, vessels supplying the axial or abaxial aspects of the medial femoral condyle were surgically interrupted in four additional juvenile miniature pigs, and the developing epiphyseal cartilage lesions were monitored using three consecutive MRI examinations over nine weeks. The miniature pigs were then euthanized, and their distal femora were harvested for histological evaluation. Vascular architecture of the distal femoral epiphyseal cartilage in the miniature pigs was found to be nearly identical to that of juvenile human subjects, characterized by separate vascular beds supplying the axial and abaxial aspects of the condyles. Surgical interruption of the vascular supply to the abaxial aspect of the medial femoral condyle resulted in ischemic cartilage necrosis (a precursor lesion of JOCD) in 75% (3/4) of the miniature pigs. Cartilage lesions were identified during the first MRI performed 3 weeks post‐operatively. No clinically apparent JOCD‐like lesions developed. In conclusion, miniature pigs are suitable for modeling JOCD precursor lesions. Further investigation of the model is warranted to assess induction of clinically apparent JOCD lesions.

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Series: Journal of orthopaedic research
ISSN: 0736-0266
ISSN-E: 1554-527X
ISSN-L: 0736-0266
Volume: 37
Issue: 10
Pages: 2130 - 2137
DOI: 10.1002/jor.24353
OADOI: https://oadoi.org/10.1002/jor.24353
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
3126 Surgery, anesthesiology, intensive care, radiology
Subjects:
Funding: NIH; Grant numbers: R01AR070020, K01OD021293,and K01AR070894; Grant sponsor: the Academy of Finland; Grant numbers: #285909, #293970, and #319440.
Copyright information: © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Tóth, F. , Johnson, C. P., Mills, B. , Nissi, M. J., Nykänen, O. , Ellermann, J. , Ludwig, K. D., Tompkins, M. and Carlson, C. S. (2019), Evaluation of the Suitability of Miniature Pigs as an Animal Model of Juvenile Osteochondritis Dissecans. J. Orthop. Res., 37: 2130-2137. doi:10.1002/jor.24353, which has been published in final form at https://doi.org/10.1002/jor.24353. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.