University of Oulu

Kauranen, H., Kinnunen, J., Hiillos, A., Lankinen, P., Hopkins, D., Wiberg, R., Ritchie, M., Hoikkala, A. (2019) Selection for reproduction under short photoperiods changes diapause-associated traits and induces widespread genomic divergence. Journal of experimental biology, 222 (20), jeb205831. doi:10.1242/jeb.205831

Selection for reproduction under short photoperiods changes diapause-associated traits and induces widespread genomic divergence

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Author: Kauranen, Hannele1; Kinnunen, Johanna1; Hiillos, Anna-Lotta1;
Organizations: 1Department of Biological and Environmental Science, University of Jyväskylä, 40014 Jyväskylä, Finland
2Department of Biology, University of Oulu, 90014 Oulu, Finland
3School of Biology, Dyers Brae House, University of St Andrews, St Andrews, KY16 9TH, UK
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 5.4 MB)
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Language: English
Published: Company of Biologists, 2019
Publish Date: 2020-10-17


The incidence of reproductive diapause is a critical aspect of life history in overwintering insects from temperate regions. Much has been learned about the timing, physiology and genetics of diapause in a range of insects, but how the multiple changes involved in this and other photoperiodically regulated traits are inter-related is not well understood. We performed quasinatural selection on reproduction under short photoperiods in a northern fly species, Drosophila montana, to trace the effects of photoperiodic selection on traits regulated by the photoperiodic timer and/or by a circadian clock system. Selection changed several traits associated with reproductive diapause, including the critical day length for diapause (CDL), the frequency of diapausing females under photoperiods that deviate from daily 24 h cycles and cold tolerance, towards the phenotypes typical of lower latitudes. However, selection had no effect on the period of free-running locomotor activity rhythm regulated by the circadian clock in fly brain. At a genomic level, selection induced extensive divergence from the control line in 16 gene clusters involved in signal transduction, membrane properties, immunologlobulins and development. These changes resembled those detected between latitudinally divergent D. montana populations in the wild and involved SNP divergence associated with several genes linked with diapause induction. Overall, our study shows that photoperiodic selection for reproduction under short photoperiods affects diapause-associated traits without disrupting the central clock network generating circadian rhythms in fly locomotor activity.

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Series: Journal of experimental biology
ISSN: 0022-0949
ISSN-E: 1477-9145
ISSN-L: 0022-0949
Volume: 222
Issue: 20
Article number: jeb205831
DOI: 10.1242/jeb.205831
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
Funding: The work has been supported by the Academy of Finland to A.H. (project 267244) and Natural Environment Research Council (NERC) funding (NE/J020818/1 to M.G.R.; NE/L501852/1 to R.A.W.W.).
Copyright information: © 2019. Published by The Company of Biologists Ltd