University of Oulu

Perevedentseva, E.; Ali, N.; Karmenyan, A.; Skovorodkin, I.; Prunskaite-Hyyryläinen, R.; Vainio, S.; Cheng, C.-L.; Kinnunen, M. Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization. Materials 2019, 12, 3762.

Optical studies of nanodiamond-tissue interaction : skin penetration and localization

Saved in:
Author: Perevedentseva, Elena1,2,3; Ali, Nsrein4; Karmenyan, Artashes1,3;
Organizations: 1Department of Physics, National Dong Hwa University, Hualien 97401, Taiwan
2P.N. Lebedev Physics Institute of Rus. Acad. Sci., Moscow 119991, Russia
3Faculty of Information Technology and Electrical Engineering, University of Oulu, 90570 Oulu, Finland
4Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, 90220 Oulu, Finland
5Borealis Biobank of Northern Finland, University of Oulu, Oulu University Hospital, 90220 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.9 MB)
Persistent link:
Language: English
Published: Multidisciplinary Digital Publishing Institute, 2019
Publish Date: 2019-12-17


In this work, several optical-spectroscopic methods have been used to visualize and investigate the penetration of diamond nanoparticles (NPs) of various sizes (3–150 nm), surface structures and fluorescence properties into the animal skin in vitro. Murine skin samples have been treated with nanodiamond (ND) water suspensions and studied using optical coherence tomography (OCT), confocal and two-photon fluorescence microscopy and fluorescence lifetime imaging (FLIM). An analysis of the optical properties of the used nanodiamonds (NDs) enables the selection of optimal optical methods or their combination for the study of nanodiamond–skin interaction. Among studied NDs, particles of 100 nm in nominal size were shown to be appropriate for multimodal imaging using all three methods. All the applied NDs were able to cross the skin barrier and penetrate the different layers of the epidermis to finally arrive in the hair follicle niches. The results suggest that NDs have the potential for multifunctional applications utilizing multimodal imaging.

see all

Series: Materials
ISSN: 1996-1944
ISSN-E: 1996-1944
ISSN-L: 1996-1944
Volume: 12
Issue: 22
Article number: 3762
DOI: 10.3390/ma12223762
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
1182 Biochemistry, cell and molecular biology
Funding: We appreciate the Academy of Finland (318103) and the Ministry of Science and Technology (MOST) of Taiwan (MOST 106-2112-M-259-009-MY3) for their financial support for the research.
Academy of Finland Grant Number: 318103
Detailed Information: 318103 (Academy of Finland Funding decision)
Copyright information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (