University of Oulu

Valery Shupletsov, Viktor Dremin, Evgeny Zherebtsov, Mikhail Mezentsev, Igor Kozlov, Elena Potapova, and Andrey Dunaev "Fluorescence spectroscopy approach for blood influence compensation", Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650R (3 June 2019);

Fluorescence spectroscopy approach for blood influence compensation

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Author: Shupletsov, Valery1; Dremin, Viktor1; Zherebtsov, Evgeny1,2;
Organizations: 1Research and Development Center of Biomedical Photonics, Orel State University named after I.S. Turgenev, Orel, Russia
2Optoelectronics and Measurement Techniques, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
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Language: English
Published: SPIE, 2019
Publish Date: 2020-01-10


At present, fluorescence spectroscopy (FS) and diffuse reflectance spectroscopy (DRS) are widespread methods highly used in medical practice. The combined application of these methods is a promising tool to improve the predictive force of classifiers for tissue type recognition as well as to compensate the attenuation of the fluorescence radiation by blood for the accurate evaluation of the biomarkers content in living tissue. Several techniques are known to normalize the resulting fluorescence spectrum in order to exclude the attenuation effect. In this study, an approach based on the dividing of the experimentally obtained FS spectrum by DRS spectrum has been applied for experiments with occlusion test. The implemented multimodal approach for the in vivo optical measurements in combination with occlusion test for minimisation of blood influence has shown good repeatability of obtained experimental fluorescence spectra. The results are of particular interest for the further development of methods for compensating the influence of chromophores in optical spectroscopy.

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Series: Progress in biomedical optics and imaging
ISSN: 1605-7422
ISSN-E: 2410-9045
ISSN-L: 1605-7422
ISBN: 978-1-5106-2821-2
ISBN Print: 978-1-5106-2820-5
Pages: 1 - 5
DOI: 10.1117/12.2522794
Host publication: Proceedings of SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, Saratov, Russian Federation, 24–28 September 2018
Host publication editor: Genina, Elina A.
Tuchin, Valery V.
Conference: International Symposium on Optics and Biophotonics
Type of Publication: A4 Article in conference proceedings
Field of Science: 213 Electronic, automation and communications engineering, electronics
217 Medical engineering
Funding: The work was funded by Russian Foundation for Basic Research according to the research project №18-02-00669.
Copyright information: © (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. Self-archived with the kind permission of the publisher.