FOXP3⁺ T cells are present in kidney biopsy samples in children with tubulointerstitial nephritis and uveitis syndrome
Rytkönen, Sari H.; Kulmala, Petri; Autio-Harmainen, Helena; Arikoski, Pekka; Endén, Kira; Kataja, Janne; Karttunen, Tuomo; Nuutinen, Matti; Jahnukainen, Timo (2017-09-11)
Rytkönen, S.H., Kulmala, P., Autio-Harmainen, H. et al. FOXP3+ T cells are present in kidney biopsy samples in children with tubulointerstitial nephritis and uveitis syndrome. Pediatr Nephrol 33, 287–293 (2018) doi:10.1007/s00467-017-3796-z
© PNA 2017. This is a post-peer-review, pre-copyedit version of an article published in Pediatric nephrology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00467-017-3796-z.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe202001131863
Tiivistelmä
Abstract
Background: Tubulointerstitial nephritis (TIN) is an inflammatory disease of unknown pathogenesis. To evaluate a possible role of regulatory T cells (Tregs) in the pathophysiology of TIN with (TINU) and without uveitis, we investigated the presence and quantity of FOXP3⁺ T regulatory lymphocytes in diagnostic kidney biopsies from pediatric patients.
Methods: A total of 33 patients (14 TIN and 19 TINU) were enrolled. The quantity of CD4⁺, FOXP3⁺ and double-positive T cells in formalin-fixed kidney biopsies was determined using double label immunohistochemistry with anti-human CD4 and FOXP3 antibodies.
Results: FOXP3 staining was successful in all 33 patients. In patients with chronic uveitis, the density of FOXP3⁺ cells was significantly lower (p = 0.046) than in TIN patients without uveitis or with uveitis lasting <3 months. CD4+ staining was successful in 23 patients. The density of all lymphocytes (CD4⁺, CD4⁺FOXP3⁺ and FOXP3⁺ cells) was significantly lower (p = 0.023) in patients with chronic uveitis than in other patients.
Conclusions: FOXP3⁺ T cells are present in kidney biopsy samples from TIN and TINU patients. In patients with chronic uveitis, the density of FOXP3⁺ T cells is significantly lower than in other patients, suggesting a different pathomechanism for these clinical conditions.
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