University of Oulu

Ivanov, V, Lee, KM, Mutanen, M. Mitonuclear discordance in wolf spiders: Genomic evidence for species integrity and introgression. Mol Ecol. 2018; 27: 1681– 1695.

Mitonuclear discordance in wolf spiders : genomic evidence for species integrity and introgression

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Author: Ivanov, Vladislav1; Lee, Kyung Min1; Mutanen, Marko1
Organizations: 1Department of Ecology and Genetics, University of Oulu, PO Box 3000, Oulu, 90014, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.9 MB)
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Language: English
Published: John Wiley & Sons, 2018
Publish Date: 2020-02-04


Systematists and taxonomists have benefited greatly from the emergence of molecular methods. Species identification has become straightforward through DNA barcoding and the rapid build‐up of massive DNA barcode reference libraries. In animals, mitonuclear discordance can significantly complicate the process of species identification and delimitation. The causes of mitonuclear discordance are either biological (e.g., introgression, incomplete lineage sorting, horizontal gene transfer androgenesis) or induced by operational factors (e.g., human error with specimen misidentification or incorrect species delimitation). Moreover, endosymbionts may play an important role in promoting fixation of mitochondrial genomes. Here, we study the mitonuclear discordance of wolf spiders species (Lycosidae) (independent cases from Alopecosa aculeata and Pardosa pullata groups) that share identical COI DNA barcodes. We approached the case utilizing double‐digest restriction site‐associated DNA sequencing (ddRADseq) to obtain and analyse genomic‐scale data. Our results suggest that the observed cases of mitonuclear discordance are not due to operational reasons but result from biological processes. Further analysis indicated introgression and that incomplete lineage sorting is unlikely to have been responsible for the observed discrepancy. Additional survey of endosymbionts provided ideas on further research and their role in shaping mitochondrial DNA distribution patterns. Thus, ddRADseq grants an efficient way to study the taxonomy of problematic groups with insight into underlying evolutionary processes.

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Series: Molecular ecology
ISSN: 0962-1083
ISSN-E: 1365-294X
ISSN-L: 0962-1083
Volume: 27
Issue: 7
Pages: 1681 - 1695
DOI: 10.1111/mec.14564
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
Funding: The whole study was funded by the Finnish Academy of Sciences, grant no. 277984 accorded to Marko Mutanen.
Academy of Finland Grant Number: 277984
Detailed Information: 277984 (Academy of Finland Funding decision)
Copyright information: © 2018 John Wiley & Sons Ltd. "This is the peer reviewed version of the following article: Ivanov, V, Lee, KM, Mutanen, M. Mitonuclear discordance in wolf spiders: Genomic evidence for species integrity and introgression. Mol Ecol. 2018; 27: 1681– 1695, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."