University of Oulu

Lauriane Janssen, Muthusamy Saranya, Mikko Leinonen, Olli Pitkänen, Ali Mobasheri, Gabriela S. Lorite, Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation, Carbon, Volume 158, 2020, Pages 681-689, ISSN 0008-6223, https://doi.org/10.1016/j.carbon.2019.11.040

Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation

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Author: Janssen, Lauriane1; Saranya, Muthusamy1; Leinonen, Mikko1;
Organizations: 1Microelectronics Research Unit, University of Oulu, Pentti Kaiteran katu 1, 90014, Oulu, Finland
2Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu 5, 08661, Vilnius, Lithuania
3Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Aapistie 5 A, 90230, Oulu, Finland
4Centre for Sport, Exercise and Osteoarthritis Research Versus Arthritis, Queen’s Medical Centre, Nottingham, NG7 2UH, United Kingdom
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe202002104954
Language: English
Published: Elsevier, 2020
Publish Date: 2021-11-14
Description:

Abstract

Articular cartilage is a highly organized tissue with very limited regenerative capacities. One limitation to mimic cartilage structure in tissue engineering is due to specific orientation of chondrocytes. Here, we use vertically aligned multi-wall carbon nanotubes (VA-MWCNT) micropillars to achieve unidirectional orientation of chondrocytes. We demonstrate that the attachment, proliferation and extracellular matrix (ECM) production by the chondrocytes is enhanced on VA-MWCNT micropillars compared to controls. The nanostructures offered by the VA-MWCNT allow the chondrocytes to anchor at cellular structure level, while mechanical flexibility of the VA-MWCNT micropillars mimics the cartilage’s natural ECM Young’s modulus. We exploit these features to extrapolate the contractile forces exerted by the chondrocytes on the micropillars. Our findings will guide the design of VA-MWCNT templates to model cell’s contractile forces. Furthermore, the capability of VA-MWCNTs to induce unidirectional chondrocytes orientation open new perspectives in cartilage tissue engineering.

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Series: Carbon. An international journal founded in conjunction with the American Carbon Society
ISSN: 0008-6223
ISSN-E: 1873-3891
ISSN-L: 0008-6223
Volume: 158
Pages: 681 - 689
DOI: 10.1016/j.carbon.2019.11.040
OADOI: https://oadoi.org/10.1016/j.carbon.2019.11.040
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
216 Materials engineering
221 Nanotechnology
Subjects:
Funding: This work was funded by Academy of Finland (#317437 and #320090).
Academy of Finland Grant Number: 317437
320090
Detailed Information: 317437 (Academy of Finland Funding decision)
320090 (Academy of Finland Funding decision)
Copyright information: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/