University of Oulu

Fouda, Shaimaa M., Gad, Mohammed M., Ellakany, Passent, Al-Thobity, Ahmad M., Al-Harbi, Fahad A., Virtanen, Jorma I., & Raustia, Aune. (2019). The effect of nanodiamonds on candida albicans adhesion and surface characteristics of PMMA denture base material - an in vitro study. Journal of Applied Oral Science, 27, e20180779. Epub November 04, 2019.

The effect of nanodiamonds on candida albicans adhesion and surface characteristics of PMMA denture base material : an in vitro study

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Author: Fouda, Shaimaa M.1; Gad, Mohammed M.1; Ellakany, Passent1;
Organizations: 1Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
2Department of Community Dentistry, Faculty of Medicine, University of Turku, Turku, Finland
3Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland
4Research Unit of Oral Health Sciences, Prosthetic Dentistry and Stomatognathic Physiology, Faculty of Medicine, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.6 MB)
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Language: English
Published: University of São Paulo, 2019
Publish Date: 2020-02-14


Candida albicans is the main causative pathogen of denture stomatitis, which affects many complete denture patients. Objective: To evaluate the effect of different concentrations of nanodiamonds (NDs) added to polymethyl methacrylate (PMMA) denture base material on Candida albicans adhesion as well as on surface roughness and contact angle. Methodology: Acrylic resin specimens sized 10×10×3 mm³ were prepared and divided into four groups (n=30) according to ND concentration (0%, 0.5%, 1%, 1.5% by wt). Surface roughness was measured with a profilometer, and the contact angle with a goniometer. The effect of NDs on Candida albicans adhesion was evaluated using two methods: 1) slide count and 2) direct culture test. Analysis of variance (ANOVA) and Tukey’s post hoc test were used in the statistical analyses. Results: Addition of NDs decreased the Candida albicans count significantly more than in the control group (p<0.05), with a lowest of 1% NDs. Addition of NDs also significantly decreased the surface roughness (p<0.05), but the contact angle remained the same. Incorporation of NDs into the PMMA denture base material effectively reduced Candida albicans adhesion and decreased surface roughness. Conclusion: PMMA/NDs composites could be valuable in the prevention of denture stomatitis, which is considered one of the most common clinical problems among removable denture wearers.

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Series: Journal of applied oral science
ISSN: 1678-7757
ISSN-E: 1678-7757
ISSN-L: 1678-7757
Volume: 27
Article number: e20180779
DOI: 10.1590/1678-7757-2018-0779
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Copyright information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.