University of Oulu

Tarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., Nazarenko, I., Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types. Small 2020, 16, 1904880.

Biodegradable nanocarriers resembling extracellular vesicles deliver genetic material with the highest efficiency to various cell types

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Author: Tarakanchikova, Yana1,2,3,4; Alzubi, Jamal5,6; Pennucci, Valentina5,6;
Organizations: 1Institute for Infection Prevention and Hospital Epidemiology, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany
2Opto‐Electronics and Measurement Techniques Research Unit, P.O. Box 4500, University of Oulu, Oulu, 90014 Finland
3Nanobiotechnology Laboratory, St. Petersburg Academic University, St. Petersburg, 194021 Russia
4RASA center in St. Petersburg, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia
5Institute for Transfusion Medicine and Gene Therapy, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany
6Center for Chronic Immunodeficiency, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany
7Department of Medicine I, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, 153000 Germany
8Department of Inorganic Chemistry, Ivanovo State University of Chemistry and Technology, Sheremetievskiy Avenue 7, 153000 Ivanovo, Russia
9Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, Infotech Oulu, University of Oulu, Borealis Biobank of Northern Finland, 138634 Oulu, Finland
10Institute of Materials Research and Engineering, A*STAR, Singapore, 138634 Singapore
11Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Saratov, Astrakhanskaya 83, 410012 Saratov, Russia
12Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET UK
13School of Life and Health Sciences, Aston University, Birmingham, B4 7ET UK
14Skoltech center of Photonics & Quantum Materials, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026 Russia
15School of Engineering and Material Science, Queen Mary University of London, London, B47ET UK
16German Cancer Consortium (DKTK), Partner Site Freiburg and German Cancer Research Center (DKFZ), Heidelberg, B47ET Germany
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.6 MB)
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Language: English
Published: John Wiley & Sons, 2020
Publish Date: 2020-02-27


Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10–4 pmol of siRNA, and 1 × 10–3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

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Series: Small
ISSN: 1613-6810
ISSN-E: 1613-6829
ISSN-L: 1613-6810
Volume: 16
Issue: 3
Article number: 1904880
DOI: 10.1002/smll.201904880
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: G.B.S. and I.N. contributed equally to this work. The work was supported by BMBF IB‐GUS/RUS 01DJ15026 and ERA‐RusPlus/ID110 Exodiagnos/BMWi ZIM collaboration project KF2979902CR4 to IN; by Government of the Russian Federation (Grant No 14.Z50.31.0004 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions and Russian institutions of higher education) to G.B.S. and D.A.G., Y.T., V.A. The authors acknowledge e‐cost Actions COST‐BM2012‐MEHAD and COST‐BM1401–Raman4Clinics. A special thanks to Bernd Giebel for providing MCSs, Jochen Mauer and Juliane Strietz for providing BCSCs, Tanja Gainey‐Schleicher and Maren Voglstaetter for technical support and to Deborah Lawrie‐Blum for proof‐reading the manuscript.
Copyright information: © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.