|
|
Biodegradable nanocarriers resembling extracellular vesicles deliver genetic material with the highest efficiency to various cell types |
|---|---|
| Author: | Tarakanchikova, Yana1,2,3,4; Alzubi, Jamal5,6; Pennucci, Valentina5,6; |
| Organizations: |
1Institute for Infection Prevention and Hospital Epidemiology, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany 2Opto‐Electronics and Measurement Techniques Research Unit, P.O. Box 4500, University of Oulu, Oulu, 90014 Finland 3Nanobiotechnology Laboratory, St. Petersburg Academic University, St. Petersburg, 194021 Russia
4RASA center in St. Petersburg, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia
5Institute for Transfusion Medicine and Gene Therapy, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany 6Center for Chronic Immunodeficiency, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany 7Department of Medicine I, Medical Center–University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, 153000 Germany 8Department of Inorganic Chemistry, Ivanovo State University of Chemistry and Technology, Sheremetievskiy Avenue 7, 153000 Ivanovo, Russia 9Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, Infotech Oulu, University of Oulu, Borealis Biobank of Northern Finland, 138634 Oulu, Finland 10Institute of Materials Research and Engineering, A*STAR, Singapore, 138634 Singapore 11Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Saratov, Astrakhanskaya 83, 410012 Saratov, Russia 12Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET UK 13School of Life and Health Sciences, Aston University, Birmingham, B4 7ET UK 14Skoltech center of Photonics & Quantum Materials, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026 Russia 15School of Engineering and Material Science, Queen Mary University of London, London, B47ET UK 16German Cancer Consortium (DKTK), Partner Site Freiburg and German Cancer Research Center (DKFZ), Heidelberg, B47ET Germany |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 1.6 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe202002276608 |
| Language: | English |
| Published: |
John Wiley & Sons,
2020
|
| Publish Date: | 2020-02-27 |
| Description: |
AbstractEfficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10–4 pmol of siRNA, and 1 × 10–3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo. see all
|
| Series: |
Small |
| ISSN: | 1613-6810 |
| ISSN-E: | 1613-6829 |
| ISSN-L: | 1613-6810 |
| Volume: | 16 |
| Issue: | 3 |
| Article number: | 1904880 |
| DOI: | 10.1002/smll.201904880 |
| OADOI: | https://oadoi.org/10.1002/smll.201904880 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
1182 Biochemistry, cell and molecular biology |
| Subjects: | |
| Funding: |
G.B.S. and I.N. contributed equally to this work. The work was supported by BMBF IB‐GUS/RUS 01DJ15026 and ERA‐RusPlus/ID110 Exodiagnos/BMWi ZIM collaboration project KF2979902CR4 to IN; by Government of the Russian Federation (Grant No 14.Z50.31.0004 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions and Russian institutions of higher education) to G.B.S. and D.A.G., Y.T., V.A. The authors acknowledge e‐cost Actions COST‐BM2012‐MEHAD and COST‐BM1401–Raman4Clinics. A special thanks to Bernd Giebel for providing MCSs, Jochen Mauer and Juliane Strietz for providing BCSCs, Tanja Gainey‐Schleicher and Maren Voglstaetter for technical support and to Deborah Lawrie‐Blum for proof‐reading the manuscript. |
| Copyright information: |
© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
|
https://creativecommons.org/licenses/by/4.0/ |