University of Oulu

Lorite, G.S., Ylä-Outinen, L., Janssen, L. et al. Carbon nanotube micropillars trigger guided growth of complex human neural stem cells networks. Nano Res. 12, 2894–2899 (2019).

Carbon nanotube micropillars trigger guided growth of complex human neural stem cells networks

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Author: Lorite, Gabriela S.1; Ylä-Outinen, Laura2; Janssen, Lauriane1;
Organizations: 1Microelectronics Research Unit, University of Oulu, PO BOX 4500, FI 90014, Finland
2NeuroGroup, BioMediTech and Faculty of Medicine and Health technology, Tampere University, Arvo Ylpön katu 34, FI 33520, Finland
3Biomaterials & Tissue Engineering Group, BioMediTech, Faculty of Medicine and Health Technology, Tampere University, PO Box 527, FI 33101, Finland
4Department of Materials Science and Nanoengineering, Rice University, P.O. Box 1892, Houston, TX 77251-1892, USA
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.2 MB)
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Language: English
Published: Springer Nature, 2019
Publish Date: 2020-02-27


New strategies for spatially controlled growth of human neurons may provide viable solutions to treat and recover peripheral or spinal cord injuries. While topography cues are known to promote attachment and direct proliferation of many cell types, guided outgrowth of human neurites has been found difficult to achieve so far. Here, three-dimensional (3D) micropatterned carbon nanotube (CNT) templates are used to effectively direct human neurite stem cell growth. By exploiting the mechanical flexibility, electrically conductivity and texture of the 3D CNT micropillars, a perfect environment is created to achieve specific guidance of human neurites, which may lead to enhanced therapeutic effects within the injured spinal cord or peripheral nerves. It is found that the 3D CNT micropillars grant excellent anchoring for adjacent neurites to form seamless neuronal networks that can be grown to any arbitrary shape and size. Apart from clear practical relevance in regenerative medicine, these results using the CNT based templates on Si chips also can pave the road for new types of microelectrode arrays to study cell network electrophysiology.

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Series: Nano research
ISSN: 1998-0124
ISSN-E: 1998-0000
ISSN-L: 1998-0124
Volume: 12
Issue: 11
Pages: 2894 - 2899
DOI: 10.1007/s12274-019-2533-2
Type of Publication: A1 Journal article – refereed
Field of Science: 3112 Neurosciences
217 Medical engineering
1182 Biochemistry, cell and molecular biology
216 Materials engineering
221 Nanotechnology
Funding: Open access funding provided by University of Oulu including Oulu University Hospital.
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