University of Oulu

I. Kestilä, E. Folkesson, M.A. Finnilä, A. Turkiewicz, P. Önnerfjord, V. Hughes, J. Tjörnstrand, M. Englund, S. Saarakkala, Three-dimensional microstructure of human meniscus posterior horn in health and osteoarthritis, Osteoarthritis and Cartilage, Volume 27, Issue 12, 2019, Pages 1790-1799, ISSN 1063-4584, https://doi.org/10.1016/j.joca.2019.07.003

Three-dimensional microstructure of human meniscus posterior horn in health and osteoarthritis

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Author: Kestilä, I.1; Folkesson, E.2,3; Finnilä, M.A.1,4;
Organizations: 1Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
2Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden
3Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Rheumatology and Molecular Skeletal Biology, Lund, Sweden
4Medical Research Center, University of Oulu, Oulu, Finland
5Lund University, Skåne University Hospital, Department of Clinical Sciences Lund, Orthopaedics, Lund, Sweden
6Clinical Epidemiology Research and Training Unit, Boston University School of Medicine, Boston, MA, USA
7Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.8 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe202003067579
Language: English
Published: Elsevier, 2019
Publish Date: 2020-03-06
Description:

Abstract

Objective: To develop and perform ex vivo 3D imaging of meniscus posterior horn microstructure using micro-computed tomography (μCT), and to compare specimens from healthy references against end-stage osteoarthritis (OA) using conventional section-based histology and qualitative μCT.

Design: We retrieved human medial and lateral menisci from 10 deceased donors without knee OA (healthy references) and medial and lateral menisci from 10 patients having total knee replacement for medial compartment OA. Meniscal posterior horns were dissected and fixed in formalin. One subsection underwent hexamethyldisilazane (HMDS) treatment and μCT imaging. Pauli's histopathological scoring was performed for 3 other subsections. The differences in histopathological scores were estimated using mixed linear regression, resulting in fixed effects estimates for within-knee comparisons and adjusted for age and body mass index for between-subjects comparisons.

Results: 3D visualization with μCT qualitatively revealed similar microstructural changes in the posterior horns as conventional histology. The mean histopathological score was higher for medial menisci from OA knees vs both medial reference menisci (mean difference [95% CI], 3.9 [2.6,5.3]), and lateral menisci from OA knees (3.9 [2.9,5.0]). The scores were similar between lateral menisci from OA knees and lateral reference menisci (0.8 [−0.6,2.2]), and between medial and lateral reference menisci (0.8 [−0.3,1.9]).

Conclusions: HMDS-based μCT protocol allows unique 3D visualization of meniscus microstructures. Posterior horns of medial menisci from medial compartment OA knees had higher histopathological scores than both the lateral posterior horns from the same OA knees and medial reference menisci, suggesting a strong association between meniscus degradation and unicompartmental knee OA.

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Series: Osteoarthritis and cartilage
ISSN: 1063-4584
ISSN-E: 1522-9653
ISSN-L: 1063-4584
Volume: 27
Issue: 12
Pages: 1790 - 1799
DOI: 10.1016/j.joca.2019.07.003
OADOI: https://oadoi.org/10.1016/j.joca.2019.07.003
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
Subjects:
Funding: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 771121) and European Research Council under the European Union's Seventh Framework Programme (FP/2007–2013) (grant agreement No 336267). This work was also supported by the Swedish Research Council (Dnr 2014–2348), the Foundation for Research in Rheumatology (FOREUM) (018EnglundPreCl), the Greta and Johan Kock Foundation, the Swedish Rheumatism Association, the Österlund Foundation, the Governmental Funding of Clinical Research program within the National Health Service (ALF) in Sweden, and the Faculty of Medicine, Lund University, Sweden.
Copyright information: © 2019 The Author(s). Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CCBY license(http://creativecommons.org/licenses/by/4.0/).
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