University of Oulu

Ukkonen, H.; Vuokila, S.; Mikkonen, J.J.W.; Dekker, H.; Schulten, E.A.J.M.; Bloemena, E.; Koistinen, A.; Valdez, T.A.; Kullaa, A.M.; Singh, S.P. Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study. Biosensors 2019, 9, 12.

Biochemical changes in irradiated oral mucosa : a FTIR spectroscopic study

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Author: Ukkonen, Helena1,2,3; Vuokila, Simo3,4; Mikkonen, Jopi J. W.3,4;
Organizations: 1Research Group of Oral Health Sciences, Faculty of Medicine, University of Oulu, and Oulu University Hospital, P.O. Box 8000, 90014 Oulu, Finland
2Medical Research Center Oulu, Oulu University Hospital, P.O. Box 5000, 90029 Oulu, Finland
3SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland
4Institute of Dentistry, University of Eastern Finland, Kuopio campus, and Educational Dental Clinic, Kuopio University Hospital, P.O. Box 1627, 70211 Kuopio, Finland
5Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
6Department of Otolaryngology, School of Medicine, Stanford University, Patlo Alto, CA 94305, USA
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.8 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2019
Publish Date: 2020-04-01


Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.

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Series: Biosensors
ISSN: 2079-6374
ISSN-E: 2079-6374
ISSN-L: 2079-6374
Volume: 9
Issue: 1
Article number: 12
DOI: 10.3390/bios9010012
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Funding: Finnish Dental Society Apollonia, The Finnish Association of Women Dentists, Orion research foundation, and Medical Research Center Oulu, funds this study to H.U.
Copyright information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (