University of Oulu

Sanna-Kaisa E. Harjula, Anni K. Saralahti, Markus J.T. Ojanen, Tommi Rantapero, Meri I.E. Uusi-Mäkelä, Matti Nykter, Olli Lohi, Mataleena Parikka, Mika Rämet, Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio), Developmental & Comparative Immunology, Volume 103, 2020, 103523, ISSN 0145-305X, https://doi.org/10.1016/j.dci.2019.103523

Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)

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Author: Harjula, Sanna-Kaisa E.1; Saralahti, Anni K.1; Ojanen, Markus J.T.1,2;
Organizations: 1Laboratory of Experimental Immunology, BioMediTech, Faculty of Medicine and Health Technology, FI-33014, Tampere University, Finland
2Laboratory of Immunoregulation, BioMediTech, Faculty of Medicine and Health Technology, FI-33014, Tampere University, Finland
3Laboratory of Computational Biology, BioMediTech, Faculty of Medicine and Health Technology, FI-33014, Tampere University, Finland
4Tampere Center for Child Health Research, Tampere University and Tays Cancer Center, Tampere University Hospital, FI-33014, Tampere University, Finland
5Laboratory of Infection Biology, BioMediTech, Faculty of Medicine and Health Technology, FI-33014, Tampere University, Finland
6Oral and Maxillofacial Unit, Tampere University Hospital, P.O. Box 2000, FI-33521, Tampere, Finland
7Department of Pediatrics, Tampere University Hospital, P.O. Box 2000, FI-33521, Tampere, Finland
8PEDEGO Research Unit, Medical Research Center Oulu, P.O. Box 8000, FI-90014, University of Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020040110060
Language: English
Published: Elsevier, 2020
Publish Date: 2020-04-01
Description:

Abstract

Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.

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Series: Developmental & comparative immunology
ISSN: 0145-305X
ISSN-E: 1879-0089
ISSN-L: 0145-305X
Volume: 103
Article number: 103523
DOI: 10.1016/j.dci.2019.103523
OADOI: https://oadoi.org/10.1016/j.dci.2019.103523
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Funding: This study was financially supported by the Academy of Finland (M.R., 277495, M.P. 316324), the Sigrid Juselius Foundation (M.R., M. P.), the Jane and Aatos Erkko Foundation (M.R., M.P.), the Competitive State Research Financing of the Expert Responsibility Area of Tampere University Hospital (M.R., M.P.), Competitive State Research Financing of the Expert Responsibility area of Oulu University Hospital (M.R.), the Tampere Tuberculosis Foundation (M.R., S.-K.H., M.P.), the University of Tampere Foundation (S.-K.H), the Emil Aaltonen Foundation (A.S., S.-K.H), Foundation of the Finnish Anti-Tuberculosis Association (S.-K.H.), the City of Tampere Science Foundation (A.S., S.-K.H), the Väinö and Laina Kivi Foundation (S.-K.H.), the Finnish Cultural Foundation, the Central Fund (S.-K.H.), the Finnish Concordia Fund (S.-K.H.), Orion Research Foundation (S.-K.H), the Academy of Finland PROFI4-project (S.-K.H.), the Finnish Society for Study of Infectious Diseases (S.-K.H.), Tampere University Doctoral Programme in Medicine and Life Sciences (A.S., M.O., M.U., T.R.), the Finnish Cultural Foundation, the Pirkanmaa Regional Fund (A.S.), the Maud Kuistila Memorial Foundation (A.S.) and the Oskar Öflunds Stiftelse (A.S.).
Academy of Finland Grant Number: 277495
Detailed Information: 277495 (Academy of Finland Funding decision)
Copyright information: © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
  https://creativecommons.org/licenses/by-nc-nd/4.0/