University of Oulu

Mauricio Rocha Dourado, Johanna Korvala, Pirjo Åström, Carine Ervolino De Oliveira, Nilva K. Cervigne, Luciana Souto Mofatto, Debora Campanella Bastos, Ana Camila Pereira Messetti, Edgard Graner, Adriana Franco Paes Leme, Ricardo D. Coletta & Tuula Salo (2019) Extracellular vesicles derived from cancer-associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma, Journal of Extracellular Vesicles, 8:1, DOI: 10.1080/20013078.2019.1578525

Extracellular vesicles derived from cancer-associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma

Saved in:
Author: Dourado, Mauricio Rocha1,2; Korvala, Johanna3; Åström, Pirjo2;
Organizations: 1Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil
2Cancer and Translational Medicine Research Unit, Faculty of Medicine and Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland
3Cancer and Translational Medicine Research Unit, Biocenter Oulu and Faculty of Medicine, University of Oulu, Oulu, Finland
4Department of Pathology and Parasitology, University of Alfenas, Alfenas, Brazil
5Department of Morphology and Basic Pathology, Faculty of Medicine of Jundiai, Jundiai, Braz
6Genomics and Expression Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Piracicaba, Brazil
7Brazilian Biosciences National Laboratory, LNBio, CNPEM, Campinas, Brazil
8Institute of Oral and Maxillofacial Disease, University of Helsinki, and HUSLAB, Department of Pathology, Helsinki University Hospital, Helsinki, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020040810763
Language: English
Published: Informa, 2019
Publish Date: 2020-04-08
Description:

Abstract

As one of the most abundant constituents of the tumour microenvironment (TME), cancer-associated fibroblasts (CAF) display critical roles during tumour progression and metastasis. Multiple classes of molecules including growth factors, cytokines, proteases and extracellular matrix proteins, are produced by CAF to act as mediators of the stroma-tumour interactions. One of the main channels for this communication is associated with extracellular vesicles (EV), which are secreted particles loaded with protein and genetic information. In this study, we evaluated the effects of EV derived from CAF primary human cell lines (n = 5) on proliferation, survival, migration, and invasion of oral squamous cell carcinoma (OSCC) cells. As controls, EV from human primary-established normal oral fibroblasts (NOF, n = 5) were used. Our in vitro assays showed that CAF-EV significantly induces migration and invasion of OSCC cells and promote a disseminated pattern of HSC-3 cell invasion in the 3D organotypic assay. Furthermore, gene expression analysis of EV-treated cancer cells revealed changes in the pathways associated with tumour metabolism and up-regulation of tumour invasion genes. Our findings suggest a significant role of CAF-EV in promoting the migration and invasion of OSCC cells, which are related to the activation of cancer-related pathways.

see all

Series: Journal of extracellular vesicles
ISSN: 2001-3078
ISSN-E: 2001-3078
ISSN-L: 2001-3078
Volume: 8
Issue: 1
Article number: 1578525
DOI: 10.1080/20013078.2019.1578525
OADOI: https://oadoi.org/10.1080/20013078.2019.1578525
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Copyright information: © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), whichpermits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  https://creativecommons.org/licenses/by-nc/4.0/