University of Oulu

Avsievich, T.; Tarakanchikova, Y.; Zhu, R.; Popov, A.; Bykov, A.; Skovorodkin, I.; Vainio, S.; Meglinski, I. Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy. Micromachines 2020, 11, 19,

Impact of nanocapsules on red blood cells interplay jointly assessed by optical tweezers and microscopy

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Author: Avsievich, Tatiana1; Tarakanchikova, Yana1,2,3; Zhu, Ruixue1;
Organizations: 1Optoelectronics and Measurement Techniques Research Unit, University of Oulu, 90014 Oulu, Finland
2Nanobiotechnology Laboratory, St. Petersburg Academic University, St. Petersburg 194021, Russia
3RASA center in St. Petersburg, Peter the Great St. Petersburg Polytechnic University, St. Petersburg 195251, Russia
4Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, University of Oulu, 90014 Oulu, Finland
5InfoTech Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, University of Oulu, 90014 Oulu, Finland
6Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk 634050, Russia
7Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University MEPhI, Moscow 115409, Russia
8Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK
9School of Life & Health Sciences, Aston University, Birmingham B4 7ET, UK
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 6.8 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2020
Publish Date: 2020-04-15


In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ’cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

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Series: Micromachines
ISSN: 2072-666X
ISSN-E: 2072-666X
ISSN-L: 2072-666X
Volume: 11
Issue: 1
Article number: 19
DOI: 10.3390/mi11010019
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: EDUFI Fellowship (TM-17-10370, TM-18-10820, T.A.), Oulun yliopiston apuraharahasto, No. 20180107, Suomen Kulttuurirahasto (grant No. 00190188, T.A.), CIMO Fellowship (TM-15-9729, Y.T.), EDUFI Fellowship (TM-17-10389, Y.T.), Academy of Finland (project no. 326126, Y.T.), China Scholarship Council (CSC No. 201706410089, R.Z.), Tauno Tönning Foundation (grant No. 20190104, R.Z.), Academy of Finland (grant No. 290596, 314369, 326126, A.B., A.P.). The authors acknowledge contribution of Russian Science Foundation (Project 19-72-30012) for the fabrication of some samples. I.M. also acknowledges partial support from the Academy of Finland (project 326204, 325097), MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005), and the National Research Tomsk State University Academic D.I. Mendeleev Fund Program.
Academy of Finland Grant Number: 326126
Detailed Information: 326126 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
314369 (Academy of Finland Funding decision)
326126 (Academy of Finland Funding decision)
326204 (Academy of Finland Funding decision)
325097 (Academy of Finland Funding decision)
Copyright information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (