University of Oulu

Mäkelä, A.M., Hohtola, E., Miinalainen, I.J. et al. Mitochondrial 2,4-dienoyl-CoA reductase (Decr) deficiency and impairment of thermogenesis in mouse brown adipose tissue. Sci Rep 9, 12038 (2019).

Mitochondrial 2,4-dienoyl-CoA reductase (Decr) deficiency and impairment of thermogenesis in mouse brown adipose tissue

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Author: Mäkelä, Anne M.1; Hohtola, Esa2; Miinalainen, Ilkka J.3;
Organizations: 1Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
2Department of Ecology and Genetics, University of Oulu, Oulu, Finland
3BCO Imaging core facilities, University of Oulu, Oulu, Finland
4Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan
5Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland
6University of Würzburg, Würzburg, Germany
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.6 MB)
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Language: English
Published: Springer Nature, 2019
Publish Date: 2020-04-21


A large number of studies have demonstrated significance of polyunsaturated fatty acids (PUFAs) for human health. However, many aspects on signals translating PUFA-sensing into body homeostasis have remained enigmatic. To shed light on PUFA physiology, we have generated a mouse line defective in mitochondrial dienoyl-CoA reductase (Decr), which is a key enzyme required for β-oxidation of PUFAs. Previously, we have shown that these mice, whose oxidation of saturated fatty acid is intact but break-down of unsaturated fatty acids is blunted, develop severe hypoglycemia during metabolic stresses and fatal hypothermia upon acute cold challenge. In the current work, indirect calorimetry and thermography suggested that cold intolerance of Decr−/− mice is due to failure in maintaining appropriate heat production at least partly due to failure of brown adipose tissue (BAT) thermogenesis. Magnetic resonance imaging, electron microscopy, mass spectrometry and biochemical analysis showed attenuation in activation of lipolysis despite of functional NE-signaling and inappropriate expression of genes contributing to thermogenesis in iBAT when the Decr−/− mice were exposed to cold. We hypothesize that the failure in turning on BAT thermogenesis occurs due to accumulation of unsaturated long-chain fatty acids or their metabolites in Decr−/− mice BAT suppressing down-stream propagation of NE-signaling.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 9
Article number: 12038
DOI: 10.1038/s41598-019-48562-x
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
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