University of Oulu

Valanne S, Salminen TS, Järvelä-Stölting M, Vesala L, Rämet M (2019) Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster. PLoS Pathog 15(1): e1007504. https://doi.org/10.1371/journal.ppat.1007504

Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster

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Author: Valanne, Susanna1; Salminen, Tiina S.1; Järvelä-Stölting, Mirva1;
Organizations: 1Laboratory of Experimental Immunology, BioMediTech Institute and Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland
2PEDEGO Research Unit, and Medical Research Center Oulu, University of Oulu, and Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland
3Department of Pediatrics, Tampere University Hospital, Tampere, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.9 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020042722583
Language: English
Published: Public Library of Science, 2019
Publish Date: 2020-04-27
Description:

Abstract

Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-κB signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism.

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Series: PLoS pathogens
ISSN: 1553-7366
ISSN-E: 1553-7374
ISSN-L: 1553-7366
Volume: 15
Issue: 1
Article number: e1007504
DOI: 10.1371/journal.ppat.1007504
OADOI: https://oadoi.org/10.1371/journal.ppat.1007504
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Funding: This work was supported by the Sigrid Juselius Foundation (http://sigridjuselius.fi/), the Academy of Finland (http://www.aka.fi/en/; grant 277495), the Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital and Tampere Tuberculosis Foundation (http://www.tuberkuloosisaatio.fi/) to MR. The work was supported by the Finnish Cultural Foundation (https://www.skr.fi/en) and Academy of Finland (http://www.aka.fi/en/; grant 276360) to LV. The Drosophila work was carried out in the Tampere Drosophila Facility, which is partly funded by Biocenter Finland (https://www.biocenter.fi/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Copyright information: © 2019 Valanne et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  https://creativecommons.org/licenses/by/4.0/