University of Oulu

Ali Mustafa Mohammed, Pasi Huuskonen, Risto Juvonen, Heidi Sahlman, Jenni Repo, Kirsi Myöhänen, Päivi Myllynen, Chit-Shing Jackson Woo, Vesa Karttunen, Kirsi Vähäkangas, Activities of metabolizing enzymes in human placenta, Toxicology Letters, Volume 326, 2020, Pages 70-77, ISSN 0378-4274, https://doi.org/10.1016/j.toxlet.2020.02.014

Activities of metabolizing enzymes in human placenta

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Author: Mohammed, Ali Mustafa1; Huuskonen, Pasi1; Juvonen, Risto1;
Organizations: 1School of Pharmacy/Toxicology, Faculty of Health Sciences, University of Eastern Finland, Finland
2Farenta Oy (a part of Oriola), Orionintie 5, Espoo, Finland
3European Chemicals Agency (ECHA), The author’s Contribution Was Done in a Personal Capacity, It Constitutes the author’s Opinion, and It Is Not an Official Position of the European Chemicals Agency (ECHA), Finland
4Department of Clinical Chemistry, Cancer and Translational Medicine Research Unit, Medical Research Center, University of Oulu and Northern Finland Laboratory Centre NordLab, Oulu University Hospital, Oulu, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2020050625348
Language: English
Published: Elsevier, 2020
Publish Date: 2021-02-27
Description:

Abstract

In addition to the transfer across the placenta, placenta displays hormonal and xenobiotic metabolism, as well as enzymatic defense against oxidative stress. We analyzed aromatase (CYP19A1), uridine 5’-diphospho-glucuronyltransferase (UGT), glutathione-S-transferase (GST) and catalase (CAT) activities in over 70 placentas from nonsmokers stored at -80 °C from former perfusion studies. A wide interindividual variation in all activities was found. Longterm storage at -80 °C did not affect the activities. Ethoxyresorufin-O-deethylase (EROD, CYP1A1) was not detected in any of the studied placentas perfused with chemicals. Several compounds in placental perfusion changed statistically significantly the enzyme activities in placental tissue. Melamine and nicotine increased CYP19A1, melamine increased UGT and GST, PhIP with ethanol decreased CYP19A1 and increased GST, and PhIP with buprenorphine decreased CAT. Antipyrine in 100 μg/ml also changed the studied enzyme activities, but not statistically significantly. Because antipyrine is a reference compound in placental perfusions, its potential effects must be taken into account in human placental perfusion. Enzyme activities deserve further studies as biomarkers of placental toxicity.

Finally, enzyme activities deserve further studies as biomarkers of placental toxicity.

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Series: Toxicology letters
ISSN: 0378-4274
ISSN-E: 1879-3169
ISSN-L: 0378-4274
Volume: 326
Pages: 70 - 77
DOI: 10.1016/j.toxlet.2020.02.014
OADOI: https://oadoi.org/10.1016/j.toxlet.2020.02.014
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Copyright information: © 2020 Published by Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/