University of Oulu

Cheddad, A., Nord, C., Hörnblad, A., Prunskaite-Hyyryläinen, R., Eriksson, M., Georgsson, F., Vainio, S., Ahlgren, U. (2013) Improving signal detection in emission optical projection tomography via single source multi-exposure image fusion. Optics Express, 21 (14), 16584-16604.

Improving signal detection in emission optical projection tomography via single source multi-exposure image fusion

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Author: Cheddad, Abbas1; Nord, Christoffer1; Hörnblad, Andreas1;
Organizations: 1Umeå Centre for Molecular Medicine, Umeå University, S-901 87 Umeå, Sweden
2Oulu Center for Cell-Matrix Research, Biocenter Oulu, Laboratory of Developmental Biology and Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Oulu, Finland
3Department of Computing Science, Umeå University, S-901 87 Umeå, Sweden
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 27.3 MB)
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Language: English
Published: Optical Society of America, 2013
Publish Date: 2020-05-08


We demonstrate a technique to improve structural data obtained from Optical Projection Tomography (OPT) using Image Fusion (IF) and contrast normalization. This enables the visualization of molecular expression patterns in biological specimens with highly variable contrast values. In the approach, termed IF-OPT, different exposures are fused by assigning weighted contrasts to each. When applied to projection images from mouse organs and digital phantoms our results demonstrate the capability of IF-OPT to reveal high and low signal intensity details in challenging specimens. We further provide measurements to highlight the benefits of the new algorithm in comparison to other similar methods.

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Series: Optics express
ISSN: 1094-4087
ISSN-E: 1094-4087
ISSN-L: 1094-4087
Volume: 21
Issue: 14
Pages: 16584 - 16604
DOI: 10.1364/OE.21.016584
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This work was supported by the Swedish research council, the European Commission (FP-7, Grant agreement no.: CP-IP 228933-2), the Kempe Foundations and by grants from Umeå University to U.A.
Copyright information: ©2013 Optical Society of America.