University of Oulu

Kaija, Helena PhDa; Pakanen, Lasse MD, PhDa,b; Porvari, Katja PhDa,c,∗ Low myocardial transcript variant alt-a of cyclin dependent kinase inhibitor p21 expression differentiates hypothermia from cardiac/respiratory causes of death, Medicine: February 2020 - Volume 99 - Issue 9 - p e19399 doi: 10.1097/MD.0000000000019399

Low myocardial transcript variant alt-a of cyclin dependent kinase inhibitor p21 expression differentiates hypothermia from cardiac/respiratory causes of death

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Author: Kaija, Helena1; Pakanen, Lasse1,2; Porvari, Katja1,3
Organizations: 1Department of Forensic Medicine, Research Unit of Internal Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu
2Forensic Medicine Unit, National Institute for Health and Welfare
3Department of Pathology, Research Unit of Cancer and Translational Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020051126032
Language: English
Published: Wolters Kluwer, 2020
Publish Date: 2020-05-11
Description:

Abstract

Gene expressions in the myocardium have been shown to vary between different causes of death, which can be utilized in the recognition of varied processes. Our previous work with a limited number of cases showed a high messenger ribonucleic acid expression of the transcript variant alt-a of cyclin dependent kinase inhibitor p21 (p21 alt-a) in chronic cardiac ischemia deaths and a low expression in hypothermia deaths and acute myocardial ischemia deaths. In present work, p21 alt-a expression in the myocardium of human cadavers was calculated using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. In this collection of 143 samples, the p21 alt-a expression was significantly lower in hypothermia than in chronic cardiac ischemic heart disease with (P < .001) or without (P < .001) acute myocardial infarction and in other cardiac and respiratory disease deaths (P < .000). Chronic ischemic heart disease in hypothermia cases did not increase the expression. The p21 alt-a expression did not correlate with postmortem interval, quality of RNA or with the age of the deceased. The p21 alt-a referenced to GAPDH expression in cadaver myocardium has apparent potential as a marker distinguishing between hypothermia and cardiac/respiratory diseases as causes of death.

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Series: Medicine
ISSN: 0025-7974
ISSN-E: 1536-5964
ISSN-L: 0025-7974
Volume: 99
Issue: 9
Article number: e19399
DOI: 10.1097/MD.0000000000019399
OADOI: https://oadoi.org/10.1097/MD.0000000000019399
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
319 Forensic science and other medical sciences
3121 General medicine, internal medicine and other clinical medicine
Subjects:
Copyright information: © 2020 the Author(s). Published by Wolters Kluwer Health, Inc. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0
  https://creativecommons.org/licenses/by-nc/4.0/