University of Oulu

Kalle Bunnfors, Natalia Abrikossova, Joni Kilpijärvi, Peter Eriksson, Jari Juuti, Niina Halonen, Caroline Brommesson, Anita Lloyd Spetz, Kajsa Uvdal, Nanoparticle activated neutrophils-on-a-chip: A label-free capacitive sensor to monitor cells at work, Sensors and Actuators B: Chemical, Volume 313, 2020, 128020, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2020.128020

Nanoparticle activated neutrophils-on-a-chip : a label-free capacitive sensor to monitor cells at work

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Author: Bunnfors, Kalle1; Abrikossova, Natalia1; Kilpijärvi, Joni1,2;
Organizations: 1Division of Molecular Surface Physics and Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83, Linköping, Sweden
2Microelectronics Research Unit, Faculty of Information Technology and Electrical Engineering, University of Oulu, P.O. Box 4500, FI-90014, University of Oulu, Finland
3Division of Sensor and Actuator Systems, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83, Linköping, Sweden
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2020051229495
Language: English
Published: Elsevier, 2020
Publish Date: 2022-03-20
Description:

Abstract

Neutrophil granulocytes are the most abundant white blood cells in mammals and vital components of the immune system. They are involved in the early phase of inflammation and in generation of reactive oxygen species. These rapid cell-signaling communicative processes are performed in the time frame of minutes.

In this work, the activity and the response of neutrophil granulocytes are monitored when triggered by cerium-oxide based nanoparticles, using capacitive sensors based on Lab-on-a-chip technology. The chip is designed to monitor activation processes of cells during nanoparticle exposure, which is for the first time recorded on-line as alteration of the capacitance. The complementary metal oxide semiconductor engineering chip design is combined with low temperature co-fired ceramic, LTCC, packaging technology. The method is label free and gently measures cells on top of an insulating surface in a weak electromagnetic field, as compared to commonly used four-point probes and impedance spectroscopy electric measurements where electrodes are in direct contact with the cells.

In summary, this label free method is used to measure oxidative stress of neutrophil granulocytes in real time, minute by minute and visualize the difference in moderate and high cellular workload during exposure of external triggers. It clearly shows the capability of this method to detect cell response during exposure of external triggers. In this way, an informationally dense non-invasive method is obtained, to monitor cells at work.

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Series: Sensors and actuators. B, Chemical
ISSN: 0925-4005
ISSN-E: 1873-3077
ISSN-L: 0925-4005
Volume: 313
Article number: 128020
DOI: 10.1016/j.snb.2020.128020
OADOI: https://oadoi.org/10.1016/j.snb.2020.128020
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Subjects:
Funding: The authors acknowledge financial support from the Swedish Research Council (Grant No. 621-2013-5357 and Grant No. 2019-02409), the Swedish Government Strategic Research Area in Materials Science on Functional Mat erials at Linköping University (Faculty Grant SFO-Mat-LiU # 2009-00971), the Knut and Alice Wallenberg Foundation through Grant No. 2012.0083CTS 18: 399, the Centre in Nano Science and Nano technology at LiTH (CeNano) at Linköping University. Part of this work was supported by the COST Action TD1105 EuNetAir "European Network on New Sensing Technologies for Air-Pollution Control and Environmental Sustainability". This work has also been financially supported by the Academy of Finland (The ClintoxNP project #268944) and TEKES (The Chempack project # 1427/31/2010). The Center of Microscopy and Nanotechnology at the University of Oulu is acknowledged for technical support.
Academy of Finland Grant Number: 268944
Detailed Information: 268944 (Academy of Finland Funding decision)
Copyright information: © 2020 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/