University of Oulu

A. Ristaniemi, J. Torniainen, L. Stenroth, M.A.J. Finnilä, T. Paakkonen, J. Töyräs, R.K. Korhonen, Comparison of water, hydroxyproline, uronic acid and elastin contents of bovine knee ligaments and patellar tendon and their relationships with biomechanical properties, Journal of the Mechanical Behavior of Biomedical Materials, Volume 104, 2020, 103639, ISSN 1751-6161, https://doi.org/10.1016/j.jmbbm.2020.103639

Comparison of water, hydroxyproline, uronic acid and elastin contents of bovine knee ligaments and patellar tendon and their relationships with biomechanical properties

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Author: Ristaniemi, A.1; Torniainen, J.1,2; Stenroth, L.1;
Organizations: 1Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
2Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland
3Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland
4School of Medicine, University of Eastern Finland, Kuopio, Finland
5School of Information Technology and Electrical Engineering, The University of Queensland, Brisbane, Australia
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2020051435612
Language: English
Published: Elsevier, 2020
Publish Date: 2022-01-17
Description:

Abstract

Mechanical material properties of ligaments originate from their biochemical composition and structural organization. However, it is not yet fully elucidated how biochemical contents vary between knee ligaments and patellar tendon (PT) and how they relate with mechanical properties. The purpose of this study was to compare water, collagen, proteoglycan and elastin contents between bovine knee ligaments and PT and correlate them with tensile material properties.

Hydroxyproline (collagen), uronic acid (proteoglycan) and elastin contents per wet and dry weights were measured using colorimetric biochemical methods for bovine knee ligament and PT samples (n = 10 knees). Direct comparison and correlation with multiple linear regression were performed against biomechanical properties measured in our earlier study.

Anterior cruciate ligament (ACL) and PT exhibited lower hydroxyproline content per wet weight compared with other ligaments (p < 0.05). Cruciate ligaments had higher uronic acid content per dry weight compared with collateral ligaments (p < 0.05). Posterior cruciate ligament had higher elastin content than ACL (p < 0.05). Higher hydroxyproline content per wet weight implied higher Young’s modulus, strength and toughness. Quantitatively, higher elastin content per wet weight predicted higher toe region nonlinearity and Young’s modulus whereas higher uronic acid content per dry weight predicted lower Young’s modulus, yield stress and toughness.

Differences between ligaments in biochemical composition highlight differences in their physiological function and loading regimes. As expected, collagen content showed similar trend with stiffness and strength. The predictive role of proteoglycan and elastin contents on the mechanical properties might indicate their important functional role in ligaments.

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Series: Journal of the mechanical behavior of biomedical materials
ISSN: 1751-6161
ISSN-E: 1878-0180
ISSN-L: 1751-6161
Volume: 104
Article number: 103639
DOI: 10.1016/j.jmbbm.2020.103639
OADOI: https://oadoi.org/10.1016/j.jmbbm.2020.103639
Type of Publication: A1 Journal article – refereed
Field of Science: 217 Medical engineering
Subjects:
Funding: We acknowledge financial support from the Academy of Finland (grants 286526 and 324529), Sigrid Juselius Foundation, Doctoral Programme in Science, Technology and Computing (SCITECO) of University of Eastern Finland, and the Research Committee of the Kuopio University Hospital Catchment Area for the State Research Funding (Project 5203111), Kuopio, Finland.
Copyright information: © 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/