University of Oulu

Joshi B, Lyngdoh S, Singh SK, Sharma R, Kumar V, Tiwari VP, et al. (2020) Revisiting the Woolly wolf (Canis lupus chanco) phylogeny in Himalaya: Addressing taxonomy, spatial extent and distribution of an ancient lineage in Asia. PLoS ONE 15(4): e0231621. https://doi.org/10.1371/journal.pone.0231621

Revisiting the Woolly wolf (Canis lupus chanco) phylogeny in Himalaya : addressing taxonomy, spatial extent and distribution of an ancient lineage in Asia

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Author: Joshi, BheemDutt1; Lyngdoh, Salvador1; Singh, Sujeet Kumar1;
Organizations: 1Wildlife Institute of India, Chandrabani, Dehradun, India
2Department of Biology, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020061543248
Language: English
Published: Public Library of Science, 2020
Publish Date: 2020-06-15
Description:

Abstract

Of the sub-species of Holarctic wolf, the Woolly wolf (Canis lupus chanco) is uniquely adapted to atmospheric hypoxia and widely distributed across the Himalaya, Qinghai Tibetan Plateau (QTP) and Mongolia. Taxonomic ambiguity still exists for this sub-species because of complex evolutionary history anduse of limited wild samples across its range in Himalaya. We document for the first time population genetic structure and taxonomic affinity of the wolves across western and eastern Himalayan regions from samples collected from the wild (n = 19) using mitochondrial control region (225bp). We found two haplotypes in our data, one widely distributed in the Himalaya that was shared with QTP and the other confined to Himachal Pradesh and Uttarakhand in the western Himalaya, India. After combining our data withpublished sequences (n = 83), we observed 15 haplotypes. Some of these were shared among different locations from India to QTP and a few were private to geographic locations. A phylogenetic tree indicated that Woolly wolves from India, Nepal, QTP and Mongolia are basal to other wolves with shallow divergence (K2P; 0.000–0.044) and high bootstrap values. Demographic analyses based on mismatch distribution and Bayesian skyline plots (BSP) suggested a stable population over a long time (~million years) with signs of recent declines. Regional dominance of private haplotypes across its distribution range may indicate allopatric divergence. This may be due to differences in habitat characteristics, availability of different wild prey species and differential deglaciation within the range of the Woolly wolf during historic time. Presence of basal and shallow divergence within-clade along with unique ecological requirements and adaptation to hypoxia, the Woolly wolf of Himalaya, QTP, and Mongolian regions may be considered as a distinct an Evolutionary Significant Unit (ESU). Identifying management units (MUs) is needed within its distribution range using harmonized multiple genetic data for effective conservation planning.

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Series: PLoS one
ISSN: 1932-6203
ISSN-E: 1932-6203
ISSN-L: 1932-6203
Issue: 15
Article number: e0231621
DOI: 10.1371/journal.pone.0231621
OADOI: https://oadoi.org/10.1371/journal.pone.0231621
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Subjects:
Funding: Wildlife Institute of India Grant in aid funds.
Dataset Reference: Supporting information:
  https://doi.org/10.1371/journal.pone.0231621.s001
https://doi.org/10.1371/journal.pone.0231621.s002
https://doi.org/10.1371/journal.pone.0231621.s003
https://doi.org/10.1371/journal.pone.0231621.s004
https://doi.org/10.1371/journal.pone.0231621.s005
https://doi.org/10.1371/journal.pone.0231621.s006
Copyright information: © 2020 Joshi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  https://creativecommons.org/licenses/by/4.0/