University of Oulu

Yu, J., Mursu, E., Typpö, M., Laaksonen, S., Voipio, H.-M., Pesonen, P., Raustia, A., & Pirttiniemi, P. (2019). MMP-3 and MMP-8 in rat mandibular condylar cartilage associated with dietary loading, estrogen level, and aging. Archives of Oral Biology, 97, 238–244.

MMP-3 and MMP-8 in rat mandibular condylar cartilage associated with dietary loading, estrogen level, and aging

Saved in:
Author: Yu, Jia1; Mursu, Eerika1; Typpö, Matleena1;
Organizations: 1Research Unit of Oral Health Sciences, University of Oulu, Oulu, Finland
2Laboratory Animal Centre, University of Oulu, Oulu, Finland
3Northern Finland Birth Cohort Studies, University of Oulu, Oulu, Finland
4Medical Research Center, Oulu University Hospital, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link:
Language: English
Published: Elsevier, 2019
Publish Date: 2019-11-01


Objectives: The structure of the mandibular condylar cartilage (MCC) is regulated by dynamic and multifactorial processes. The aim of this study was to examine the effects of altered dietary loading, estrogen level, and aging on the structure of the condylar cartilage and the expressions of matrix metalloproteinase (MMP) -3 and MMP-8 of rat MCC.

Methods: In this study, Crl:CD (SD) female rats were randomly divided into 3 groups according to dietary hardness: hard diet (diet board), normal diet (pellet), and soft diet (powder). In each group, the rats were further divided into 2 subgroups by ovariectomy at the age of 7 weeks. The rats were sacrificed at 5- and 14-month-old. Histomorphometric analysis of the MCC thickness was performed after toluidine blue staining. Immunochemical staining was done for MMP-3 and MMP-8. A linear mixed model was used to assess the effects of dietary loading, estrogen level, and aging. Results: Increased dietary loading was the main factor to increase the MMP-3 expression and the anterior and central thickness of the MCC. Lack of estrogen was the main factor associated with decreased MMP-8. Aging was associated with the thickness changes of the whole condylar cartilage and the reduced expression of MMP-8.

Conclusion: The condylar cartilage structure and metabolism of the female rats are sensitive to dietary loading changes, estrogen level as well as aging. The proper balance of these factors seems to be essential for the maintenance of the condylar cartilage.

see all

Series: Archives of oral biology
ISSN: 0003-9969
ISSN-E: 1879-1506
ISSN-L: 0003-9969
Volume: 97
Pages: 238 - 244
DOI: 10.1016/j.archoralbio.2018.10.037
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Funding: This study was supported by the Chinese Scholarship Council fellowship (201608450067).
Copyright information: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http:/