University of Oulu

Gennadii Piavchenko, Alexander Alekseev, Olga Stelmashchuk, Evgeniya Seryogina, Evgeny Zherebtsov, Elena Kuznetsova, Andrey Dunaev, Yuri Volkov, Sergey Kuznetsov, A complex morphofunctional approach for zinc toxicity evaluation in rats, Heliyon, Volume 6, Issue 4, 2020, e03768, ISSN 2405-8440,

A complex morphofunctional approach for zinc toxicity evaluation in rats

Saved in:
Author: Piavchenko, Gennadii1,2,3; Alekseev, Alexander3; Stelmashchuk, Olga3;
Organizations: 1I.M. Sechenov First Moscow State Medical University (Sechenov University), Russian Federation
2Pharmaceutical R&D Enterprise “Retinoids”, Russian Federation
3Orel State University named after I.S. Turgenev, Russian Federation
4University of Oulu, Finland
5School of Medicine and Trinity Translational Medicine Institute, The University of Dublin, Trinity College, Ireland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.8 MB)
Persistent link:
Language: English
Published: Elsevier, 2020
Publish Date: 2020-07-03


Anthropogenic activity causes the introduction of zinc compounds into the biological cycle in mining and processing sites and its accumulation in organs and tissues, causing systemic toxicity. A cumulative effect of zinc is predominantly neurotoxic and it also affects the respiratory, cardiovascular and digestive systems. This study evaluates the effects of single-dose intragastric administration of 100 mg/kg zinc succinate on the structure and function of organs and tissues in male Wistar rats 1 month after treatment. The presented morphofunctional approach for the toxicity evaluation included the study of behavioral responses using the automated Laboras® complex, fluorescent spectral analysis of the NADH and FAD activity and histological evaluation of animal organs and tissues. The results of the behavioral activity assessment showed a significant decrease in animals’ motor activity, whereas the fluorescence spectra analysis demonstrated a decrease in coenzyme NADH without the reduction of FAD levels. We detected toxic and dystrophic changes in the cerebral cortex, heart, lungs and liver tissues. Our original multiparametric approach enables a comprehensive assessment of the long-term toxic effects of the metal salts such as zinc succinate, especially in the cerebral cortex at the doses much lower than the acute LD50 reported for the common zinc salts.

see all

Series: Heliyon
ISSN: 2405-8440
ISSN-E: 2405-8440
ISSN-L: 2405-8440
Volume: 6
Issue: 4
Article number: e03768
DOI: 10.1016/j.heliyon.2020.e03768
Type of Publication: A1 Journal article – refereed
Field of Science: 3121 General medicine, internal medicine and other clinical medicine
Copyright information: © 2020 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (