Abstract

During the last century, the human footprint on natural ecosystems has increased strongly and human-altered habitats such as urban and agricultural areas have extended globally. Despite their negative impacts on biodiversity, these habitats offer unique opportunities to study how native species respond to novel environmental conditions. Here, we studied phenotypic divergence associated with colonization of human-altered habitats in the Speckled wood (Pararge aegeria). We reared butterflies of woodland, urban and agricultural origins under common garden conditions and we measured boldness and activity at the adult stage. Both behavioural traits were repeatable at the individual level (i.e. personality traits), but we found weak evidence for ecotype-related differences in mean boldness and activity. In line with urban areas being stressful habitats, we found that boldness and activity traits correlate in urban butterflies, while we found no such syndrome in woodland and agricultural butterflies. Our results show that urbanization can alter some aspects of personality in an insect species, but they do not support the prediction that anthropogenic habitats favour boldness.