University of Oulu

Tuominen, H., Al-Samadi, A., Salo, T. et al. Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells. Virol J 17, 87 (2020). https://doi.org/10.1186/s12985-020-01367-1

Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells

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Author: Tuominen, Heidi1,2; Al-Samadi, Ahmed3,4; Salo, Tuula3,4,5,6,7;
Organizations: 1Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Lemminkäisenkatu 2, FIN-20520, Turku, Finland
2Department of Pathology, Turku University Hospital, Turku, Finland
3Translational Immunology Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland
4Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland
5Medical Research Center Oulu, Oulu University Hospital, Oulu, Finland
6Cancer Research and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland
7HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020081860988
Language: English
Published: Springer Nature, 2020
Publish Date: 2020-08-18
Description:

Abstract

Background/Objectives: This study was designed to investigate the invasion of human papillomavirus (HPV) positive human cervical carcinoma cell lines in human leiomyoma-based extracellular matrices in vitro, and to test the suitability of the model for studying the irradiation effects on the cancer cell invasion.

Methods: HPV positive cervical carcinoma cell lines SiHa and CaSki, and HPV negative squamous cell carcinoma cell line HSC-3 were used. CaSki cells contain around 600 copies of HPV 16 virus in the genome, whereas SiHa have only 1–2 copies per cell. Cells were analyzed using two different human tumor derived extracellular matrix methods (3D myoma disc model, and Myogel Transwell invasion assay). Cultures were irradiated with 4 Gy. Myoma invasion area and the depth of invasion were measured with ImageJ 1.51j8 software. Statistical analyses were performed with SPSS Statistics (IBM SPSS® Statistics 25).

Results: All cells invaded through Myogel coated Transwell membranes and within myoma discs. In myoma discs, a difference in the invasion depth (p = 0.0001) but not in invasion area (p = 0.310) between the HPV positive cell lines was seen, since SiHa (less HPV) invaded slightly better than CaSki (more HPV). HSC-3 cells (HPV negative) invaded deepest (p = 0.048) than either of the HPV positive cell line cells. No difference was detected in the invasion area (p = 0.892) between HPV positive and HPV negative cells. The ionized radiation significantly reduced the invasion depth of HSC-3 (p = 0.008), SiHa (p = 0.0001) and CaSki (p = 0.005). No significant effect on the invasion area was detected in any of the cell lines. However, a significant difference was observed between SiHa and CaSki in the reduction of the invasion depth after radiation (p = 0.013) as the reduction was greater with SiHa than CaSki.

Conclusions: Both solid and gelatinous human leiomyoma-based extracellular matrix models were suitable platforms to study the invasion of HPV positive cervical carcinoma cells in vitro. SiHa cells with less HPV copy number cells invaded slightly better and were slightly more sensitive to irradiation than CaSki cells with high HPV copy number. However, there was no drastic differences between the invasion properties of these carcinoma cells.

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Series: Virology journal
ISSN: 1743-422X
ISSN-E: 1743-422X
ISSN-L: 1743-422X
Volume: 17
Article number: 87
DOI: 10.1186/s12985-020-01367-1
OADOI: https://oadoi.org/10.1186/s12985-020-01367-1
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
HPV
Funding: This work was supported by research grants from by the Finnish Doctoral Programme in Oral Sciences (FINDOS), Cancer Foundation in South-Western Finland, Dental Association of Turku, Finnish Dental Society Apollonia, Finnish Women Dentists’ Association and Minerva Foundation. The funders had no role in study design, data collection and analyses, decision to publish or preparation of the manuscript.
Copyright information: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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