University of Oulu

Virpiranta, H.; Banasik, M.; Taskila, S.; Leiviskä, T.; Halttu, M.; Sotaniemi, V.-H.; Tanskanen, J. Isolation of Efficient Metal-Binding Bacteria from Boreal Peat Soils and Development of Microbial Biosorbents for Improved Nickel Scavenging. Water 2020, 12, 2000.

Isolation of efficient metal-binding bacteria from boreal peat soils and development of microbial biosorbents for improved nickel scavenging

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Author: Virpiranta, Hanna1; Banasik, Michal2; Taskila, Sanna1;
Organizations: 1Chemical Process Engineering, University of Oulu, PO Box 4300, 90014 Oulu, Finland
2Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. Narutowicza 11/12, 80-233 Gdańsk, Poland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.8 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2020
Publish Date: 2020-08-20


Boreal peatlands with low iron availability are a potential, but rarely studied, source for the isolation of bacteria for applications in metal sorption. The present research focused on the isolation and identification of Actinobacteria from northern Finland, which can produce siderophores for metal capture. The 16S rDNA analysis showed that isolated strains belonged to Firmicutes (Bacillus sp.) and Actinobacteria (Microbacterium sp.). The culture most efficiently producing siderophores in the widest array of the media was identified as Microbacterium sp. The most appropriate media for siderophore production by the Microbacterium strain were those prepared with glucose supplemented with asparagine or glutamic acid, and those prepared with glycerol or fructose supplemented with glutamic acid. The microorganism obtained and its siderophores were used to develop Sphagnum moss-based hybrid biosorbents. It was showed that the hybrid sorbent could bind nickel ions and that the nickel removal was enhanced by the presence of siderophores. Bacterial cells did not have a significant effect on sorption efficiency compared to the use of siderophores alone. The microbial biosorbent could be applied in the final effluent treatment stage for wastewater with low metal concentrations.

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Series: Water
ISSN: 2073-4441
ISSN-E: 2073-4441
ISSN-L: 2073-4441
Volume: 12
Issue: 7
Article number: 2000
DOI: 10.3390/w12072000
Type of Publication: A1 Journal article – refereed
Field of Science: 219 Environmental biotechnology
Funding: This research was funded by the European Regional Development Fund, project A71699 “Sustainable refining of peatland biomasses to valuable products”. The study was also part of the “Supporting Environmental, Economic and Social Impacts of Mining Activity” (KO1030 SEESIMA) research project and received financial support from the Kolarctic CBC (Cross-Border Collaboration), the European Union, Russia, Norway, Finland, and Sweden. Its contents are the sole responsibility of the authors at the University of Oulu, and do not necessarily reflect the views of the European Union or the participating countries. The sequencing costs were covered by the financing of Maa- ja vesitekniikan tuki (MVTT) in the project entitled “Microbes in constructed forest wetlands as biocatalysts in wastewater treatment”.
Copyright information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (