Daddali R, Ojaniemi M, Hallman M, Rämet M, Haapalainen AM (2020) CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. PLoS ONE 15(6): e0234403. https://doi.org/10.1371/journal.pone.0234403
CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery
|Author:||Daddali, Ravindra1,2; Ojaniemi, Marja1,2; Hallman, Mikko1,2;|
1PEDEGO Research Unit and Medical Research Center Oulu, University of Oulu, Oulu, Finland
2Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland
3BioMediTech, Institute and Faculty of Medical and Life Sciences, University of Tampere, Tampere, Finland
|Online Access:||PDF Full Text (PDF, 1.3 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2020090165977
Public Library of Science,
|Publish Date:|| 2020-09-01
MicroRNAs (miRNAs) are important regulators of gene expression, and their expression is associated with many physiological conditions. Here, we investigated potential associations between expression levels of miRNAs in human placenta and the onset of spontaneous term birth. Using RNA sequencing, we identified 54 miRNAs differentially expressed during spontaneous term labor compared to elective term births. Expression levels of 23 miRNAs were upregulated, whereas 31 were downregulated at least 1.5-fold. The upregulated miRNA miR-371a-5p putatively targets CPPED1, expression of which decreases during spontaneous birth. We used a luciferase reporter–based assay to test whether a miR-371a-5p mimic affected translation when it bound to the 3′ untranslated region of CPPED1. In this setting, the miR-371a-5p mimic resulted in lower luciferase activity, which suggests that miR-371a-5p regulates levels of CPPED1. In conclusion, inversely correlated levels of miR-371a-5p and CPPED1 suggest a role for both in spontaneous delivery.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
This work was supported by the Jane and Aatos Erkko Foundation (MH and MR), Sigrid Jusélius Foundation (MH), Competitive State Research Financing of the Expert Responsibility Area of Oulu University Hospital (MR), and Pediatric Research Foundation (AMH).
© 2020 Daddali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.