University of Oulu

Kajabi, AW, Casula, V, Ojanen, S, et al. Multiparametric MR imaging reveals early cartilage degeneration at 2 and 8 weeks after ACL transection in a rabbit model. J Orthop Res. 2020; 38: 1974– 1986. https://doi.org/10.1002/jor.24644

Multiparametric MR imaging reveals early cartilage degeneration at 2 and 8 weeks after ACL transection in a rabbit model

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Author: Kajabi, Abdul Wahed1,2; Casula, Victor1,2; Ojanen, Simo1,3;
Organizations: 1Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland
2Medical Research Center Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland
3Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
4Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada
5Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020090969011
Language: English
Published: John Wiley & Sons, 2020
Publish Date: 2020-09-09
Description:

Abstract

In this study, the rabbit model with anterior cruciate ligament transection (ACLT) was used to investigate early degenerative changes in cartilage using multiparametric quantitative magnetic resonance imaging (qMRI). ACLT was surgically induced in the knees of skeletally mature New Zealand White rabbits (n = 14). ACL transected and contralateral knee compartments—medial femur, lateral femur, medial tibia, and lateral tibia—were harvested 2 (n = 8) and 8 weeks (n = 6) postsurgery. Twelve age‐matched nonoperated rabbits served as control. qMRI was conducted at 9.4 T and included relaxation times T₁, T₂, continuous‐wave T1p (CWT1p), adiabatic T1p (AdT1p), adiabatic T2p (AdT2p), and relaxation along a fictitious field (TRAFF). For reference, quantitative histology and biomechanical measurements were carried out. Posttraumatic changes were primarily noted in the superficial half of the cartilage. Prolonged T₁, T₂, CWT1p, and AdT1p were observed in the lateral femur 2 and 8 weeks post‐ACLT, compared with the corresponding control and contralateral groups (P < .05). Collagen orientation was significantly altered in the lateral femur at 2 weeks post‐ACLT compared with the corresponding control group. In the medial femur, all the studied relaxation time parameters, except TRAFF, were increased 8 weeks post‐ACLT, as compared with the corresponding contralateral and control groups (P < .05). Similarly, significant proteoglycan loss was observed in the medial femur at 8 weeks following surgery (P < .05). Multiparametric MRI demonstrated early degenerative changes primarily in the superficial cartilage with T₁, T₂, CWT1p, and AdT1p sensitive to cartilage changes at 2 weeks after surgery.

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Series: Journal of orthopaedic research
ISSN: 0736-0266
ISSN-E: 1554-527X
ISSN-L: 0736-0266
Volume: 38
Issue: 9
Pages: 1974 - 1986
DOI: 10.1002/jor.24644
OADOI: https://oadoi.org/10.1002/jor.24644
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
3111 Biomedicine
Subjects:
MRI
Funding: This study was supported by Jane and Aatos Erkko Foundation, the Academy of Finland (grants #297033, #285909, #293970, #286526, #268378, and #303786), European Research Council under the European Union's Seventh Framework Programme (FP/2007‐2013, ERC grant #336267), Sigrid Juselius Foundation, Saastamoinen Foundation, Päivikki ja Sakari Sohlberg Foundation, Finnish Cultural Foundation North Savo Regional Fund, the Canadian Institutes for Health Research, the Canada research Chair Programme and the Killam Foundation. The funding sources had no role in the study design, collection, analysis, interpretation of data, in writing of the manuscript, or in decision to submit the manuscript for publication.
Academy of Finland Grant Number: 297033
285909
293970
286526
268378
303786
Detailed Information: 297033 (Academy of Finland Funding decision)
285909 (Academy of Finland Funding decision)
293970 (Academy of Finland Funding decision)
286526 (Academy of Finland Funding decision)
268378 (Academy of Finland Funding decision)
303786 (Academy of Finland Funding decision)
Copyright information: © 2020 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
  https://creativecommons.org/licenses/by-nc/4.0/