Multiparametric MR imaging reveals early cartilage degeneration at 2 and 8 weeks after ACL transection in a rabbit model |
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Author: | Kajabi, Abdul Wahed1,2; Casula, Victor1,2; Ojanen, Simo1,3; |
Organizations: |
1Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland 2Medical Research Center Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland 3Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
4Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada
5Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland |
Format: | article |
Version: | published version |
Access: | open |
Online Access: | PDF Full Text (PDF, 4.2 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2020090969011 |
Language: | English |
Published: |
John Wiley & Sons,
2020
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Publish Date: | 2020-09-09 |
Description: |
AbstractIn this study, the rabbit model with anterior cruciate ligament transection (ACLT) was used to investigate early degenerative changes in cartilage using multiparametric quantitative magnetic resonance imaging (qMRI). ACLT was surgically induced in the knees of skeletally mature New Zealand White rabbits (n = 14). ACL transected and contralateral knee compartments—medial femur, lateral femur, medial tibia, and lateral tibia—were harvested 2 (n = 8) and 8 weeks (n = 6) postsurgery. Twelve age‐matched nonoperated rabbits served as control. qMRI was conducted at 9.4 T and included relaxation times T₁, T₂, continuous‐wave T1p (CWT1p), adiabatic T1p (AdT1p), adiabatic T2p (AdT2p), and relaxation along a fictitious field (TRAFF). For reference, quantitative histology and biomechanical measurements were carried out. Posttraumatic changes were primarily noted in the superficial half of the cartilage. Prolonged T₁, T₂, CWT1p, and AdT1p were observed in the lateral femur 2 and 8 weeks post‐ACLT, compared with the corresponding control and contralateral groups (P < .05). Collagen orientation was significantly altered in the lateral femur at 2 weeks post‐ACLT compared with the corresponding control group. In the medial femur, all the studied relaxation time parameters, except TRAFF, were increased 8 weeks post‐ACLT, as compared with the corresponding contralateral and control groups (P < .05). Similarly, significant proteoglycan loss was observed in the medial femur at 8 weeks following surgery (P < .05). Multiparametric MRI demonstrated early degenerative changes primarily in the superficial cartilage with T₁, T₂, CWT1p, and AdT1p sensitive to cartilage changes at 2 weeks after surgery. see all
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Series: |
Journal of orthopaedic research |
ISSN: | 0736-0266 |
ISSN-E: | 1554-527X |
ISSN-L: | 0736-0266 |
Volume: | 38 |
Issue: | 9 |
Pages: | 1974 - 1986 |
DOI: | 10.1002/jor.24644 |
OADOI: | https://oadoi.org/10.1002/jor.24644 |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
3126 Surgery, anesthesiology, intensive care, radiology 3111 Biomedicine |
Subjects: | |
Funding: |
This study was supported by Jane and Aatos Erkko Foundation, the Academy of Finland (grants #297033, #285909, #293970, #286526, #268378, and #303786), European Research Council under the European Union's Seventh Framework Programme (FP/2007‐2013, ERC grant #336267), Sigrid Juselius Foundation, Saastamoinen Foundation, Päivikki ja Sakari Sohlberg Foundation, Finnish Cultural Foundation North Savo Regional Fund, the Canadian Institutes for Health Research, the Canada research Chair Programme and the Killam Foundation. The funding sources had no role in the study design, collection, analysis, interpretation of data, in writing of the manuscript, or in decision to submit the manuscript for publication. |
Academy of Finland Grant Number: |
297033 285909 293970 286526 268378 303786 |
Detailed Information: |
297033 (Academy of Finland Funding decision) 285909 (Academy of Finland Funding decision) 293970 (Academy of Finland Funding decision) 286526 (Academy of Finland Funding decision) 268378 (Academy of Finland Funding decision) 303786 (Academy of Finland Funding decision) |
Copyright information: |
© 2020 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
https://creativecommons.org/licenses/by-nc/4.0/ |