University of Oulu

Jeremiasse, B., Matta, C., Fellows, C.R. et al. Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines. BMC Mol and Cell Biol 21, 47 (2020). https://doi.org/10.1186/s12860-020-00288-9

Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines

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Author: Jeremiasse, Bernadette1; Matta, Csaba2; Fellows, Christopher R.3;
Organizations: 1Department of Rheumatology & Clinical Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands
2Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
3Department of Veterinary Pre-Clinical Sciences, School of Veterinary Science and Medicine, University of Surrey, Guildford, UK
4John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, NG11 8NS, UK
5Bruker UK Limited, Coventry, UK
6Exonate Ltd., Medicity, Thane Road, Nottingham, UK
7Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
8Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
9Centre for Sport, Exercise and Osteoarthritis Research Versus Arthritis, Queen’s Medical Centre, Nottingham, UK
10Department of Orthopedics, UMC Utrecht, Utrecht, The Netherlands
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.6 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020091169349
Language: English
Published: Springer Nature, 2020
Publish Date: 2020-09-11
Description:

Abstract

Chondrocytes are exposed to an inflammatory micro-environment in the extracellular matrix (ECM) of articular cartilage in joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). In OA, degenerative changes and low-grade inflammation within the joint transform the behaviour and metabolism of chondrocytes, disturb the balance between ECM synthesis and degradation, and alter the osmolality and ionic composition of the micro-environment. We hypothesize that chondrocytes adjust their physiology to the inflammatory microenvironment by modulating the expression of cell surface proteins, collectively referred to as the ‘surfaceome’. Therefore, the aim of this study was to characterize the surfaceome of primary equine chondrocytes isolated from healthy joints following exposure to the pro-inflammatory cytokines interleukin-1-beta (IL-1β) and tumour necrosis factor-alpha (TNF-α). We employed combined methodology that we recently developed for investigating the surfaceome in stem cells. Membrane proteins were isolated using an aminooxy-biotinylation technique and analysed by mass spectrometry using high throughput shotgun proteomics. Selected proteins were validated by western blotting.

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Series: BMC molecular and cell biology
ISSN: 2661-8850
ISSN-E: 2661-8850
ISSN-L: 2661-8850
Volume: 21
Issue: 1
Article number: 47
DOI: 10.1186/s12860-020-00288-9
OADOI: https://oadoi.org/10.1186/s12860-020-00288-9
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
Funding: AM was the co-ordinator of the D-BOARD Consortium funded by European Commission Framework 7 programme (EU FP7; HEALTH.2012.2.4.5–2, project number 305815, Novel Diagnostics and Biomarkers for Early Identification of Chronic Inflammatory Joint Diseases). AM has received funding from the Deanship of Scientific Research (DSR), King AbdulAziz University (grant no. 1–141/1434 HiCi). AM is a member of the Arthritis Research UK Centre for Sport, Exercise, and Osteoarthritis, funded by Arthritis Research UK (Grant Reference Number: 20194). AM was funded by the European Social Fund according to the activity ‘Improvement of researchers’ qualification by implementing world-class R&D projects of Measure No. 09.3.3-LMT-K-712 (grant application code: 09.3.3-LMT-K-712-01-0157, agreement No. DOTSUT-215). CM was supported by the European Commission through a Marie Skłodowska-Curie Intra-European Fellowship for career development (project number: 625746; acronym: CHONDRION; FP7-PEOPLE-2013-IEF), and by the Premium Postdoctoral Research Fellowship of the Hungarian Academy of Sciences. CM also received support from the Bridging Fund of the University of Debrecen, Faculty of Medicine, and from the Thematic Excellence Programme of the Ministry for Innovation and Technology in Hungary, within the framework of the Space Sciences thematic programme of the University of Debrecen (ED 18-1-2019-0028). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data, and in writing the manuscript.
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