University of Oulu

Irina N. Novikova, Andreea Manole, Evgeny A. Zherebtsov, Dmitry D. Stavtsev, Marina N. Vukolova, Andrey V. Dunaev, Plamena R. Angelova, Andrey Y. Abramov, Adrenaline induces calcium signal in astrocytes and vasoconstriction via activation of monoamine oxidase, Free Radical Biology and Medicine, Volume 159, 2020, Pages 15-22, ISSN 0891-5849, https://doi.org/10.1016/j.freeradbiomed.2020.07.011

Adrenaline induces calcium signal in astrocytes and vasoconstriction via activation of monoamine oxidase

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Author: Novikova, Irina N.1; Manole, Andreea2; Zherebtsov, Evgeny A.1,3;
Organizations: 1Cell Physiology and Pathology Laboratory, Orel State University, Orel, 302026, Russia
2Salk Institute for Biological Studies, CA, 92037, USA
3Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, 90014, Finland
4Department of Pathophysiology, Sechenov First Moscow State Medical University, Moscow, 119991, Russia
5Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, Queens Square, London, WC1N 3BG, UK
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2020100176318
Language: English
Published: Elsevier, 2020
Publish Date: 2021-07-30
Description:

Abstract

Adrenaline or epinephrine is a hormone playing an important role in physiology. It is produced de-novo in the brain in very small amounts compared to other catecholamines, including noradrenaline. Although the effects of adrenaline on neurons have been extensively studied, much less is known about the action of this hormone on astrocytes. Here, we studied the effects of adrenaline on astrocytes in primary co-culture of neurons and astrocytes. Application of adrenaline induced calcium signal in both neurons and astrocytes, but only in neurons this effect was dependent on α- and β-receptor antagonists. The effects of adrenaline on astrocytes were less dependent on adrenoreceptors: the antagonist carvedilol had only moderate effect on the calcium signal and the agonist of adrenoreceptors methoxamine induced a signal only in small proportion of the cells. We found that adrenaline in astrocytes activates phospholipase C and subsequent release of calcium from the endoplasmic reticulum. Calcium signal in astrocytes is initiated by the metabolism of adrenaline by the monoamine oxidase (MAO), which activates reactive oxygen species production and induces lipid peroxidation. Inhibitor of MAO selegiline inhibited both adrenaline-induced calcium signal in astrocytes and the vasoconstriction that indicates an important role for monoamine oxidase in adrenaline-induced signalling and function.

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Series: Free radical biology & medicine
ISSN: 0891-5849
ISSN-E: 1873-4596
ISSN-L: 0891-5849
Volume: 159
Pages: 15 - 22
DOI: 10.1016/j.freeradbiomed.2020.07.011
OADOI: https://oadoi.org/10.1016/j.freeradbiomed.2020.07.011
Type of Publication: A1 Journal article – refereed
Field of Science: 217 Medical engineering
Subjects:
Funding: This work was supported by the grant of the Russian Federation Government no. 075- 15-2019-1877 and EPSRC grant
Copyright information: © 2020 Elsevier Inc. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/