University of Oulu

Ali, N., Rezvani, H.R., Motei, D. et al. Trisk 95 as a novel skin mirror for normal and diabetic systemic glucose level. Sci Rep 10, 12246 (2020).

Trisk 95 as a novel skin mirror for normal and diabetic systemic glucose level

Saved in:
Author: Ali, Nsrein1,2; Rezvani, Hamid Reza3,4; Motei, Diana1;
Organizations: 1Laboratory of Developmental Biology, Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, Aapistie 5A, 90220, Oulu, Finland
2Infotech Oulu, University of Oulu, 90220, Oulu, Finland
3Inserm, BMGIC, UMR 1035, University of Bordeaux, Bordeaux, France
4Centre de Référence pour les Maladies Rares de la Peau, CHU de Bordeaux, Bordeaux, France
5Inserm U1216, Grenoble Institut des Neurosciences, University Grenoble, La Tronche, France
6Biocenter Oulu, University of Oulu, 90220, Oulu, Finland
7Borealis Biobank of Northern Finland, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.5 MB)
Persistent link:
Language: English
Published: Springer Nature, 2020
Publish Date: 2020-10-07


Developing trustworthy, cost effective, minimally or non-invasive glucose sensing strategies is of great need for diabetic patients. In this study, we used an experimental type I diabetic mouse model to examine whether the skin would provide novel means for identifying biomarkers associated with blood glucose level. We first showed that skin glucose levels are rapidly influenced by blood glucose concentrations. We then conducted a proteomic screen of murine skin using an experimental in vivo model of type I diabetes and wild-type controls. Among the proteins that increased expression in response to high blood glucose, Trisk 95 expression was significantly induced independently of insulin signalling. A luciferase reporter assay demonstrated that the induction of Trisk 95 expression occurs at a transcriptional level and is associated with a marked elevation in the Fluo-4AM signal, suggesting a role for intracellular calcium changes in the signalling cascade. Strikingly, these changes lead concurrently to fragmentation of the mitochondria. Moreover, Trisk 95 knockout abolishes both the calcium flux and the mitochondrial phenotype changes indicating dependency of glucose flux in the skin on Trisk 95 function. The data demonstrate that the skin reacts robustly to systemic blood changes, and that Trisk 95 is a promising biomarker for a glucose monitoring assembly.

see all

Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 10
Issue: 1
Article number: 12246
DOI: 10.1038/s41598-020-68972-6
Type of Publication: A1 Journal article – refereed
Field of Science: 3121 General medicine, internal medicine and other clinical medicine
Funding: This work was supported Academy of Finland Grants (251314, 315030, 307533, 206038, 121647), Finnish Cultural, Sigrid Jusélius and Finnish Cancer Research Foundations, the European Community's Seventh Framework Programme Health (FP7/2007-2013) under Grant Agreement FP7-HEALTH-F5-2012-INNOVATION-1 EURenOmics 305608, and H2020-FETOPEN-2018-019-2020-01 projects MindGap GA 829040 and Gladiator (GA 828837).
EU Grant Number: (305608) EURENOMICS - European Consortium for High-Throughput Research in Rare Kidney Diseases
(829040) MindGAP - Bridging the gap between Mind, Brain and Body: exosome role and monitoring
(828837) GLADIATOR - Next-generation theranostics of brain pathologies with autonomous externally controllable nanonetworks: a trans-disciplinary approach with bio-nanodevice interfaces
Academy of Finland Grant Number: 251314
Detailed Information: 251314 (Academy of Finland Funding decision)
315030 (Academy of Finland Funding decision)
307533 (Academy of Finland Funding decision)
206038 (Academy of Finland Funding decision)
121647 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit