University of Oulu

Vallittu, PK, Posti, JP, Piitulainen, JM, et al. Biomaterial and implant induced ossification: in vitro and in vivo findings. J Tissue Eng Regen Med. 2020; 14: 1157– 1168.

Biomaterial and implant induced ossification : in vitro and in vivo findings

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Author: Vallittu, Pekka K.1; Posti, Jussi P.2; Piitulainen, Jaakko M.3;
Organizations: 1Department of Biomaterials Science, Institute of Dentistry, University of Turku and City of Turku, Welfare Division, Turku, Finland
2Division of Clinical Neurosciences, Department of Neurosurgery, Turku Brain Injury Centre, Turku University Hospital and University of Turku, Turku, Finland
3Division of Surgery and Cancer Diseases, Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, Turku Finland and University of Turku, Turku, Finland
4PEDEGO Research Unit, University of Oulu, Oulu, Finland and Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland
5Institute of Biomedicine, University of Turku, Turku, Finland
6International Clinical Research Center of St. Anne's University Hospital Brno, Brno, Czech Republic
7Department of Oral Pathology and Radiology, Institute of Dentistry, University of Turku, Turku, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.5 MB)
Persistent link:
Language: English
Published: John Wiley & Sons, 2020
Publish Date: 2020-11-09


Material‐induced ossification is suggested as a suitable approach to heal large bone defects. Fiber‐reinforced composite–bioactive glasses (FRC‐BGs) display properties that could enhance the ossification of calvarial defects. Here, we analyzed the healing processes of a FRC‐BG implant in vivo from the perspective of material‐induced ossification. Histological analysis of the implant, which was removed 5 months after insertion, showed the formation of viable, noninflammatory mesenchymal tissue with newly‐formed mineralized woven bone, as well as nonmineralized connective tissue with capillaries and larger blood vessels. The presence of osteocytes was detected within the newly generated bone matrix. To expand our understanding on the osteogenic properties of FRC‐BG, we cultured human adipose tissue‐derived mesenchymal stromal cells (AD‐MSCs) in the presence of two different BGs (45S5 and S53P4) and Al2O3 control. AD‐MSCs grew and proliferated on all the scaffolds tested, as well as secreted abundant extracellular matrix, when osteogenic differentiation was appropriately stimulated. 45S5 and S53P4 induced enhanced expression of COL2A1, COL10A1, COL5A1 collagen subunits, and pro‐osteogenic genes BMP2 and BMP4. The concomitant downregulation of BMP3 was also detected. Our findings show that FRC‐BG can support the vascularization of the implant and the formation of abundant connective tissue in vivo. Specifically, BG 45S5 and BG S53P4 are suited to evoke the osteogenic potential of host mesenchymal stromal cells. In conclusion, FRC‐BG implant demonstrated material‐induced ossification both in vitro and in vivo.

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Series: Journal of tissue engineering and regenerative medicine
ISSN: 1932-6254
ISSN-E: 1932-7005
ISSN-L: 1932-6254
Volume: 14
Issue: 8
Pages: 1157 - 1168
DOI: 10.1002/term.3056
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
3126 Surgery, anesthesiology, intensive care, radiology
Funding: Giancarlo Forte and Stefania Pagliari were supported by the European Social Fund and European Regional Development Fund‐Project MAGNET (CZ.02.1.01/0.0/0.0/15_003/0000492). The study was supported by the BioCity Turku Biomaterials and Medical Device Research Program ( FRC scaffolds for MSC tests were provided by Skulle Implants Corporation ( Jussi P. Posti is supported by the Academy of Finland (grant #17379) and Pekka K. Vallittu and Jorma Määttä are supported by the Academy of Finland (grant #323596).
Copyright information: © 2020 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.