Blood flow visualization by means of laser speckle-contrast measurements under the conditions of nonergodicity |
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Author: | Sdobnov, A. Yu.1; Kalchenko, V. V.2; Bykov, A. V.1; |
Organizations: |
1University of Oulu, Optoelectronics and Measurement Techniques Laboratory, Oulu, 90570 Finland 2Weizmann Institute of Science, Department of Veterinary Resources, Rehovot, 76100 Israel 3Interdisciplinary Laboratory for Biophotonics, National Research Tomsk State University, Tomsk, 634050 Russia
4Institute of Engineering Physics for Biomedicine, National Research Nuclear University (MEPhI), Moscow, 115409 Russia
5Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET, UK 6School of Life and Health Sciences, Aston University, B4 7ET Birmingham, UK |
Format: | article |
Version: | accepted version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.9 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2020111189957 |
Language: | English |
Published: |
Pleiades Publishing,
2020
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Publish Date: | 2020-11-11 |
Description: |
AbstractThe influence of stationary structural inclusions in inhomogeneous strongly scattering media such as biotissues on the results of speckle-contrast measurements using temporal and spatial methods of image processing by means of laser speckle-contrast imaging is investigated. The limits of applicability of the method of laser speckle-contrast imaging are established in detail for the case in which ergodicity conditions are not fulfilled. Based on model experiments, it is demonstrated that an increase in the number of stationary scatterers relative to that of the dynamic ones in the sampling volume introduces substantial error into the results of spatial and temporal processing of speckle images for given exposure time of the detector. At the same time, analysis of spatial and temporal speckle contrasts, the values of the coefficient of speckle dynamics, along with the results of Monte-Carlo simulation of the sampling volume, revealed that the presence of a relatively thin, up to 30% of entire volume, static layer does not introduce considerable changes into the results of measurements by the method of laser speckle-contrast imaging. The exposure time of the camera, along with the number of frames used for image processing, can be varied and chosen individually for each experiment. The developed algorithms of spatial and temporal processing of images obtained by the method of laser speckle-contrast imaging were tested in the experiments on transcranial visualization of the cerebral blood flow of a mouse. see all
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Series: |
Optics and spectroscopy |
ISSN: | 0030-400X |
ISSN-E: | 1562-6911 |
ISSN-L: | 0030-400X |
Volume: | 128 |
Issue: | 6 |
Pages: | 778 - 786 |
DOI: | 10.1134/S0030400X2006020X |
OADOI: | https://oadoi.org/10.1134/S0030400X2006020X |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
114 Physical sciences |
Subjects: | |
Funding: |
A.S. acknowledges the support of the Finnish Cultural Foundation (grant no. 00180998). I.M. acknowledges partial support of the Academy of Finland (grants nos. 326204 and 325097), NEUROPA Research and Innovation Program Horizon 2020 (project no. 863214), MEPhI Academic Excellence Project no. 232 02.a03.21.0005, and the funding program of D.I. Mendeleev Tomsk State University. A.B. acknowledges the support of the Academy of Finland (grant no. 290596). A.P. acknowledges the support of the Academy of Finland (grant no. 314369) and the INFOTECH project. V.K. acknowledges the support of the Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics (Staff Scientists grant program). |
EU Grant Number: |
(863214) NEUROPA - Non-invasive dynamic neural control by laser-based technology |
Academy of Finland Grant Number: |
314369 326204 325097 290596 |
Detailed Information: |
314369 (Academy of Finland Funding decision) 326204 (Academy of Finland Funding decision) 325097 (Academy of Finland Funding decision) 290596 (Academy of Finland Funding decision) |
Copyright information: |
© The Author(s), 2020, published in Optika i Spektroskopiya. Self-archived here in accordance with the guidelines set by the publisher. The Definitive Version of Record can be found online at: https://doi.org/10.1134/S0030400X2006020X. |