University of Oulu

Sdobnov, A.Y., Kalchenko, V.V., Bykov, A.V. et al. Blood Flow Visualization by Means of Laser Speckle-Contrast Measurements under the Conditions of Nonergodicity. Opt. Spectrosc. 128, 778–786 (2020).

Blood flow visualization by means of laser speckle-contrast measurements under the conditions of nonergodicity

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Author: Sdobnov, A. Yu.1; Kalchenko, V. V.2; Bykov, A. V.1;
Organizations: 1University of Oulu, Optoelectronics and Measurement Techniques Laboratory, Oulu, 90570 Finland
2Weizmann Institute of Science, Department of Veterinary Resources, Rehovot, 76100 Israel
3Interdisciplinary Laboratory for Biophotonics, National Research Tomsk State University, Tomsk, 634050 Russia
4Institute of Engineering Physics for Biomedicine, National Research Nuclear University (MEPhI), Moscow, 115409 Russia
5Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET, UK
6School of Life and Health Sciences, Aston University, B4 7ET Birmingham, UK
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.9 MB)
Persistent link:
Language: English
Published: Pleiades Publishing, 2020
Publish Date: 2020-11-11


The influence of stationary structural inclusions in inhomogeneous strongly scattering media such as biotissues on the results of speckle-contrast measurements using temporal and spatial methods of image processing by means of laser speckle-contrast imaging is investigated. The limits of applicability of the method of laser speckle-contrast imaging are established in detail for the case in which ergodicity conditions are not fulfilled. Based on model experiments, it is demonstrated that an increase in the number of stationary scatterers relative to that of the dynamic ones in the sampling volume introduces substantial error into the results of spatial and temporal processing of speckle images for given exposure time of the detector. At the same time, analysis of spatial and temporal speckle contrasts, the values of the coefficient of speckle dynamics, along with the results of Monte-Carlo simulation of the sampling volume, revealed that the presence of a relatively thin, up to 30% of entire volume, static layer does not introduce considerable changes into the results of measurements by the method of laser speckle-contrast imaging. The exposure time of the camera, along with the number of frames used for image processing, can be varied and chosen individually for each experiment. The developed algorithms of spatial and temporal processing of images obtained by the method of laser speckle-contrast imaging were tested in the experiments on transcranial visualization of the cerebral blood flow of a mouse.

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Series: Optics and spectroscopy
ISSN: 0030-400X
ISSN-E: 1562-6911
ISSN-L: 0030-400X
Volume: 128
Issue: 6
Pages: 778 - 786
DOI: 10.1134/S0030400X2006020X
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
Funding: A.S. acknowledges the support of the Finnish Cultural Foundation (grant no. 00180998). I.M. acknowledges partial support of the Academy of Finland (grants nos. 326204 and 325097), NEUROPA Research and Innovation Program Horizon 2020 (project no. 863214), MEPhI Academic Excellence Project no. 232 02.a03.21.0005, and the funding program of D.I. Mendeleev Tomsk State University. A.B. acknowledges the support of the Academy of Finland (grant no. 290596). A.P. acknowledges the support of the Academy of Finland (grant no. 314369) and the INFOTECH project. V.K. acknowledges the support of the Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics (Staff Scientists grant program).
EU Grant Number: (863214) NEUROPA - Non-invasive dynamic neural control by laser-based technology
Academy of Finland Grant Number: 314369
Detailed Information: 314369 (Academy of Finland Funding decision)
326204 (Academy of Finland Funding decision)
325097 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
Copyright information: © The Author(s), 2020, published in Optika i Spektroskopiya. Self-archived here in accordance with the guidelines set by the publisher. The Definitive Version of Record can be found online at: